Time-resolved single-cell sequencing identifies multiple waves of mRNA decay during the mitosis-to-G1 phase transition
| Autor: | Stijn Sonneveld, Lenno Krenning, Marvin E Tanenbaum |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
General Immunology and Microbiology
Sequence Analysis RNA QH301-705.5 RNA Stability General Neuroscience Science Cell Biology General Medicine single-cell sequencing Chromosomes and Gene Expression General Biochemistry Genetics and Molecular Biology Cell Line HEK293 Cells Gene Expression Regulation mRNA decay Humans Medicine cell cycle Biology (General) gene regulation Transcription Factors Research Article Human |
| Zdroj: | eLife, Vol 11 (2022) eLife |
| Popis: | Accurate control of the cell cycle is critical for development and tissue homeostasis, and requires precisely timed expression of many genes. Cell cycle gene expression is regulated through transcriptional and translational control, as well as through regulated protein degradation. Here, we show that widespread and temporally controlled mRNA decay acts as an additional mechanism for gene expression regulation during the cell cycle in human cells. We find that two waves of mRNA decay occur sequentially during the mitosis-to-G1 phase transition, and we identify the deadenylase CNOT1 as a factor that contributes to mRNA decay during this cell cycle transition. Collectively, our data show that, akin to protein degradation, scheduled mRNA decay helps to reshape cell cycle gene expression as cells move from mitosis into G1 phase. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|