Photodynamic effect of hypericin in primary cultures of human umbilical endothelial cells and glioma cell lines
| Autor: | Alexander Ostro, Jan Mojzis, Andrej Mirossay, Marek Šarišský, Ladislav Mirossay, Robert Dankovcik, Viktória Stupáková, Lenka Varinská, Peter Urdzík |
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| Rok vydání: | 2009 |
| Předmět: |
Cell type
Angiogenesis medicine.medical_treatment Photodynamic therapy DNA Fragmentation Biology chemistry.chemical_compound Cell Movement Glioma Cell Line Tumor medicine Humans Cytotoxicity Perylene Pharmacology Anthracenes Photosensitizing Agents Neovascularization Pathologic Endothelial Cells medicine.disease Hypericin Endothelial stem cell chemistry Matrix Metalloproteinase 9 Photochemotherapy Cell culture Gelatinases Immunology Cancer research Matrix Metalloproteinase 2 |
| Zdroj: | Phytotherapy research : PTR. 23(6) |
| ISSN: | 1099-1573 |
| Popis: | Hypericin is the most powerful naturally occurring photosensitizer and as such there is renaissant interest in the potentials of this compound for anticancer photodynamic therapy (PDT). The purpose of this study was to investigate the hypericin-mediated photodynamic therapy effects on normal human umbilical endothelial cells (HUVECs) in comparison with cancer human glioma cell lines U-87 MG and U-373 MG, in in vitro conditions. The data suggest that endothelial cells as well as glioma cell lines are sensitive only to photoactivated hypericin. The inhibitory effects of photoactivated hypericin did not differ in endothelial compared with tumor cells in cytotoxicity MTT and DNA fragmentation assays. However, an important difference in sensitivity was found between the above mentioned cell types in migration and metalloproteinases inhibition assays performed as cell function tests. The findings in both function tests were supported by the high sensitivity of endothelial cells in an additional angiogenesis test of tubular formation in vitro. |
| Databáze: | OpenAIRE |
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