Sequence relationships between plasmids associated with conventional MLS resistance and zonal lincomycin resistance in Streptococcus pyogenes
| Autor: | Werner Reichardt, Artem A. Totolian, Iontova Im, A.S. Boitsov, Golubkov Vi, Horst Malke |
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| Rok vydání: | 1982 |
| Předmět: |
DNA
Bacterial Streptococcus pyogenes Inverted repeat DNA Recombinant Biology medicine.disease_cause Virginiamycin law.invention Restriction fragment chemistry.chemical_compound Plasmid 23S ribosomal RNA law Genetics medicine Molecular Biology Repetitive Sequences Nucleic Acid Base Sequence Drug Resistance Microbial DNA Restriction Enzymes Molecular biology Lincomycin carbohydrates (lipids) Microscopy Electron chemistry Recombinant DNA biology.protein DNA Plasmids medicine.drug |
| Zdroj: | Molecular and General Genetics MGG. 187:310-315 |
| ISSN: | 1432-1874 0026-8925 |
| DOI: | 10.1007/bf00331135 |
| Popis: | By using electron microscopy of self-annealed DNA and restriction enzyme analysis, we have compared the physical maps of two group A streptococcal plasmids associated with conventional MLS resistance (pEL1; 20 Md) and zonal lincomycin resistance (pSM10419; 15 Md). Of their monomeric molecules, about 40% and 60%, respectively, are occupied by identical non-tandem inverted repeats containing sequences specifying putative replication functions. Sequence homology also exists between their resistance determinants which are located in unique DNA. Moreover, homology between additional regions of unknown function is so extensive and restriction fragment arrangement so similar that, formally, pSM10419 can be considered a deletion variant of pEL1. The results suggest that MLS and zonal lincomycin resistance have the same biochemical basis (i.e. methylation of 23S ribosomal RNA) and differ only quantitatively in the inducible control systems. |
| Databáze: | OpenAIRE |
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