Mapping of the Pim-1 oncogene in mouse t-haplotypes and its use to define the relative map positions of the tcl loci t0(t6) and tw12 and the marker tf (tufted)
| Autor: | Dorothea Bennett, Gregory Gummere, Karen Artzt, Belinda Ark |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Genetic Markers
Recombination Genetic Genetics Chromosome Mapping Locus (genetics) Oncogenes Biology Genetic analysis law.invention Chromosome 17 (human) Blotting Southern Mice Haplotypes Gene mapping law hemic and lymphatic diseases Recombinant DNA Animals Lethal allele Gene Polymorphism Restriction Fragment Length Southern blot |
| Zdroj: | Genomics. 10:385-389 |
| ISSN: | 0888-7543 |
| DOI: | 10.1016/0888-7543(91)90323-7 |
| Popis: | Pim-1 is an oncogene activated in mouse T-cell lymphomas induced by Moloney and AKR mink cell focus (MCF) viruses. Pim-1 was previously mapped to chromosome 17 by somatic cell hybrids, and subsequently to the region between the hemoglobin α-chain pseudogene 4 ( Hba-4ps ) and the α-crystalline gene ( Crya-1 ) by Southern blot analysis of DNA obtained from panels of recombinant inbred strains. We have now mapped Pim-1 more accurately in t -haplotypes by analysis of recombinant t -chromosomes. The recombinants were derived from Tt s6 tf t 12 parents backcrossed to + tf +tf , and scored for recombination between the loci of T and tf . For simplicity all t -complex lethal genes properly named tck-t x are shortened to t x . The Pim-1 gene was localized 0.6 cM proximal to the t w 12 lethal gene, thus placing the Pim-1 gene 5.2 cM distal to the H-2 region in t -haplotypes. Once mapped, the Pim-1 gene was used as a marker for further genetic analysis of t -haplotypes, t w 12 is so close to tf that even with a large number of recombinants it was not possible to determine whether it is proximal or distal to tf . Southern blot analysis of DNA from T-tf recombinants with a separation of t w 12 and tf indicated that t w 12 is proximal to tf . The mapping of two alletic t -lethals, t 0 and t 6 with respect to t w 12 and tf has also been a problem. The map position of t 0 ( t 6 ) was obtained through Southern blot analysis of DNA from a panel of Tt s6 tf t 0 and Tt s6 tf t 6 recombinants with separation of t w 12 and t 0 ( t 6 ). t 0 ( t 6 ) was placed distal to tf . Southern blot analysis of the t h 20 deletion, which covers the locus of tf , indicated that this deletion does not include the Pim-1 gene. |
| Databáze: | OpenAIRE |
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