In vitro investigation of methylene blue-bearing, electrostatically assembled aptamer–silica nanocomposites as potential photodynamic therapeutics
| Autor: | Hsin-Yun Hsu, Xin Chun Huang, Yun Ling Luo, Tian Shyng Ding |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Cell Survival
Silicon dioxide medicine.medical_treatment Aptamer Static Electricity Photodynamic therapy Photochemistry Nanocomposites chemistry.chemical_compound Drug Delivery Systems Colloid and Surface Chemistry Cell Line Tumor Neoplasms medicine Humans Photosensitizer Physical and Theoretical Chemistry chemistry.chemical_classification Reactive oxygen species Photosensitizing Agents Nanocomposite Singlet Oxygen Singlet oxygen Mucin-1 Surfaces and Interfaces General Medicine Silicon Dioxide Methylene Blue Photochemotherapy chemistry Adsorption Hydrophobic and Hydrophilic Interactions Methylene blue Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 135:217-224 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2015.07.064 |
| Popis: | Photodynamic therapy, that is, excitation of a photosensitizer with light to generate reactive oxygen species such as singlet oxygen, has emerged as a noninvasive technique for cancer theranostics. However, the clinical use of many photosensitizers is impeded by their hydrophobicity, the nonspecific damage they cause to normal tissues, and their susceptibility to environmental degradation. In this study, we developed a simple electrostatic adsorption strategy to fabricate aptamer-silica nanocomposites by sequentially functionalizing nanocomposites with the cell surface-associated mucin 1 aptamer for tumor targeting and a hydrophilic photosensitizer, methylene blue, for photodynamic therapy applications. We investigated the relationship between the biophysical properties and cellular uptake of such nanocomposites to improve their formulation. Effective generation of singlet oxygen was achieved with a low photosensitizer dosage (0.5 μM) and a short, low-power irradiation (1 min, 10 mW/cm(2)). With the current strategy, the efficiency of photodynamic therapy was determined by the cellular uptake of nanocomposites and the targeting molecules used. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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