Effect of training and growth hormone suppression on insulin-like growth factor I mRNA in young rats
| Autor: | D. Leroith, Jo Anne Brasel, M. Y. Moromisato, S. Zanconato, D. M. Cooper, D. Y. Moromisato, C. T. Roberts, John R. Woods |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
INSULIN-LIKE GROWTH FACTOR I PROTEIN LEVEL
medicine.medical_specialty LIVER Anabolism Physiology medicine.medical_treatment HINDLIMB MUSCLE Muscle Proteins Gene Expression Hindlimb Biology Medical and Health Sciences Rats Sprague-Dawley Insulin-like growth factor Exon Ribonucleases Physiology (medical) Internal medicine Physical Conditioning Animal Gene expression medicine Animals RNA Messenger Insulin-Like Growth Factor I Growth factor Muscles Skeletal muscle Nucleic Acid Hybridization Biological Sciences Actins Rats Endocrinology medicine.anatomical_structure Liver Growth Hormone Autoradiography Female Hormone |
| Zdroj: | Zanconato, S; Moromisato, DY; Moromisato, MY; Woods, J; Brasel, JA; Leroith, D; et al.(1994). Effect of training and growth hormone suppression on insulin-like growth factor I mRNA in young rats.. Journal of applied physiology (Bethesda, Md. : 1985), 76(5), 2204-2209. doi: 10.1152/jappl.1994.76.5.2204. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/1mm2w3cn Scopus-Elsevier |
| DOI: | 10.1152/jappl.1994.76.5.2204. |
| Popis: | The growth hormone (GH)-insulin-like growth factor I (IGF-I) axis plays a role in the adaptation to exercise training, but IGF-I gene expression in response to exercise training and GH suppression has not been studied. Twenty female rates underwent a 4-wk treadmill training program begun in the prepubertal period (day 14 of life). In 10 of the training rats, GH production was suppressed by anti-GH-releasing hormone antibodies (GH suppressed). IGF-I mRNA and protein levels were measured in liver and hindlimb skeletal muscle. GH suppression reduced IGF-I mRNA expression in the liver to a much greater extent than in the muscle. In the GH control rats, training induced significant increases in hepatic exon 1-derived IGF-I mRNA (mean increase 30%; P < 0.05) and muscle exon 2-derived mRNA (mean increase 35%; P < 0.05). In the GH-suppressed rats, only muscle exon 1-derived transcripts were significantly increased by training (55%; P < 0.05) and this was associated with a significant increase in muscle IGF-I protein levels (P < 0.05). We speculate that the anabolic response to training may involve both GH-dependent increases in IGF-I mRNA in the liver and GH-independent increases in the muscle. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|