Phosphonated chelates for nuclear imaging
| Autor: | Sabah Abada, Laurence Ehret-Sabatier, Ali Ouadi, Patrice Laquerriere, David Brasse, Falk Saupe, Mourad Elhabiri, Loïc J. Charbonnière, Alexandre Lecointre, Câline Christine, Thomas Hussenet, Gertraud Orend |
|---|---|
| Přispěvatelé: | Département Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Institut Pluridisciplinaire Hubert Curien (IPHC), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Immuno-Rhumatologie Moléculaire, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Strasbourg (UNISTRA), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Substances naturelles/chimie moléculaire, Université Louis Pasteur - Strasbourg I-Ecole européenne de chimie, polymères et matériaux [Strasbourg]-Centre National de la Recherche Scientifique (CNRS), Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'innovation moléculaire et applications (LIMA), Centre National de la Recherche Scientifique (CNRS)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université de Strasbourg (UNISTRA) |
| Rok vydání: | 2014 |
| Předmět: |
Tris
Biodistribution diagnosis analysis Pyridines Reducing agent Stereochemistry Aucun Organophosphonates Breast Neoplasms Mice Transgenic chemistry Ligands Physique [physics]/Physique [physics] Biochemistry Dissociation (chemistry) Mice chemistry.chemical_compound Coordination Complexes Animals [CHIM]Chemical Sciences Tissue Distribution Chelation Physical and Theoretical Chemistry Bifunctional Chelating Agents Tomography Emission-Computed Single-Photon [PHYS]Physics [physics] [PHYS.PHYS]Physics [physics]/Physics [physics] Organic Chemistry Antibodies Monoclonal Tenascin Rats 3. Good health Thermodynamics Female Titration Radiopharmaceuticals Cyclic voltammetry Nuclear chemistry |
| Zdroj: | Organic and Biomolecular Chemistry Organic and Biomolecular Chemistry, Royal Society of Chemistry, 2014, 12 (47), pp.9601-9620. ⟨10.1039/c4ob01514b⟩ Organic and Biomolecular Chemistry, Royal Society of Chemistry, 2014, 12 (47), pp.9601-9620. ⟨10.1039/C4OB01514B⟩ |
| ISSN: | 1477-0539 1477-0520 |
| DOI: | 10.1039/c4ob01514b |
| Popis: | A series of bis-, tris- and tetra-phosphonated pyridine ligands is presented. In view of their potential use as chelates for radiopharmaceutical applications, the physico-chemical properties of the ligands and of their Co(II), Ni(II), Cu(II), and Zn(II) complexes were studied by means of potentiometry and UV-Vis absorption spectroscopy. The pKa values of the ligands and of the complexes, as well as the stability constants for the formation of the complexes, are presented. The kinetic aspects of the formation of Cu(II) complexes and of their dissociation in acidic media were studied by means of stopped flow experiments, and the stability of the Cu(II) complex toward reduction to Cu(I) was investigated by cyclic voltammetry and by titration with different reducing agents. The different thermodynamic and kinetic aspects of the polyphosphonated ligands were compared with regard to the impact of the number of phosphonic acid functions. Considering the very promising properties for complexation, preliminary SPECT/CT imaging experiments were carried out on mice with (99m)Tc using the bis- and tetra-phosphonated ligands L(2) and L(1). Finally, a bifunctional version of chelate L(1), L*, was used to label MTn12, a rat monoclonal antibody with both specificity and relatively high affinity for murine tenascin-C. The labeling was monitored by MALDI/MS spectrometry and the affinity of the labeled antibody was checked by immunostaining experiments. After chelation with (99m)Tc, the (99m)Tc-L*-MTn12 antibody was injected into a transgenic mouse with breast cancer and the biodistribution of the labeled antibody was followed by SPECT/CT imaging. journal article research support, non-u.s. gov't 2014 Dec 21 2014 10 23 imported |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|