Essential genes of the macrophage response to Staphylococcus aureus exposure
Autor: | Jianzhong Chen, Aixia Sun, Hongwei Zhang, Jian Zhang, Guifen Niu, Feng Pang, Fei Chen |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Staphylococcus aureus medicine.diagnostic_test Macrophage lcsh:Cytology Cell Biology Biology medicine.disease_cause Biochemistry Molecular medicine Microbiology 03 medical and health sciences 030104 developmental biology Real-time polymerase chain reaction Western blot CEBPA Gene expression Bio-informatics medicine lcsh:QH573-671 Molecular Biology Gene |
Zdroj: | Cellular & Molecular Biology Letters, Vol 23, Iss 1, Pp 1-14 (2018) |
ISSN: | 1689-1392 1425-8153 |
Popis: | Background Although significant advances have been made in understanding the mechanisms of macrophage response to Staphylococcus aureus infection, the molecular details are still elusive. Identification of the essential genes and biological processes of macrophages that are specifically changed at different durations of S. aureus exposure is of great clinical significance. Methods We aimed to identify the significantly changed genes and biological processes of S. aureus-exposed macrophages. We systematically analyzed the macrophage gene expression profile GSE 13670 database with 8 h, 24 h or 48 h S. aureus infection. The results were further confirmed by western blot and quantitative polymerase chain reaction (qPCR) analyses. Results After 8 h of S. aureus infection, the expression of 624 genes was significantly changed. Six hundred thirteen differentially expressed genes (DEGs) were identified after 24 h of S. aureus infection. Two hundred fifty-three genes were significantly changed after 48 h of S. aureus infection. STAT1 was consistently up-regulated in these three treatments. TP53, JAK2, CEBPA, STAT3, MYC, CTNNB1 and PRKCA were only identified in the 8 h or 24 h S. aureus infection groups. CTNNB1 and PRKCA were for the first time identified as potential essential genes in S. aureus infection of macrophages. In the Gene Ontology (GO) term analysis, the defense response was shown to be the most significantly changed biological process among all processes; KEGG pathway analysis identified the JAK-STAT signaling pathway involved in early infection. Conclusions Our systematic analysis identified unique gene expression profiles and specifically changed biological processes of the macrophage response to different S. aureus exposure times. |
Databáze: | OpenAIRE |
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