Tandem duplications resulting from recombination between ribosomal RNA genes in Escherichia coli
| Autor: | Barry S. Hillman, Charles W. Hill, Robert H. Grafstrom, Barbara Wallis Harnish |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
DNA
Bacterial Genetic Linkage Mutant Biology medicine.disease_cause Structural Biology Gene duplication Escherichia coli medicine A-DNA Molecular Biology Gene Chromosome Aberrations Recombination Genetic Genetics DNA Superhelical Chromosomes Bacterial Ribosomal RNA Microscopy Electron Genes RNA Ribosomal Mutation Tandem exon duplication DNA Circular Recombination |
| Zdroj: | Journal of Molecular Biology. 116:407-428 |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/0022-2836(77)90077-8 |
| Popis: | A set of Escherichia coli K12 mutants, which carry a tandem duplication of the glyT purD region, have been analyzed. Three types of duplications have occurred repeatedly, and we show that they were generated by recombination between the ribosomal RNA gene, rrnE, which lies to one side of the glyT purD region and one of threerrn genes which occur as direct repetitions on the other side of this region. Characterization of these duplication mutants has involved the isolation of the duplicated material in the form of a DNA circle. Class I duplications, which extend from rrnE to rrnE, are 39,500 base-pairs long, class II duplications, which extend from rrnA to rrnE, are 164,000 base-pairs long, and class III duplications, which extend from rrnC to rrnE, are 258,000 base-pairs long. |
| Databáze: | OpenAIRE |
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