Gene profile of zebrafish fin regeneration offers clues to kinetics, organization and biomechanics of basement membrane
| Autor: | Marilyne Malbouyres, Pauline Nauroy, Florence Ruggiero, Sandrine Hughes, Benjamin Gillet, Julien Chlasta, Elise Lambert, Alexandre Guiraud |
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| Přispěvatelé: | Laboratoire de Biologie Moléculaire de la Cellule (LBMC), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Matrice extracellulaire et régulations cellulaires (MERC), Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Inflammasome NLRP3 – NLRP3 Inflammasome, Centre International de Recherche en Infectiologie - UMR (CIRI), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), ANR-16-CE18-0023,Healskin,Matrices pour la régénération et la cicatrisation cutanée(2016) |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Basement membrane [SDV]Life Sciences [q-bio] Collagen XIV Fin regeneration Extracellular matrix 03 medical and health sciences 0302 clinical medicine Gene expression medicine Extracellular Animals Regeneration Molecular Biology Zebrafish ComputingMilieux_MISCELLANEOUS Extracellular Matrix Proteins Wound Healing biology Chemistry Regeneration (biology) Gene Expression Regulation Developmental Zebrafish Proteins biology.organism_classification Embryonic stem cell Cell biology Kinetics 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Animal Fins Collagen CRISPR-Cas Systems Transcriptome |
| Zdroj: | Matrix Biology Matrix Biology, Elsevier, 2019, 75-76, pp.82-101. ⟨10.1016/j.matbio.2018.07.005⟩ Matrix Biology, 2019, 75-76, pp.82-101. ⟨10.1016/j.matbio.2018.07.005⟩ |
| ISSN: | 0945-053X |
| Popis: | International audience; How some animals regenerate missing body parts is not well understood. Taking advantage of the zebrafish caudal fin model, we performed a global unbiased time-course transcriptomic analysis of fin regeneration. Biostatistics analyses identified extracellular matrix (ECM) as the most enriched gene sets. Basement membranes (BMs) are specialized ECM structures that provide tissues with structural cohesion and serve as a major extracellular signaling platform. While the embryonic formation of BM has been extensively investigated, its regeneration in adults remains poorly studied. We therefore focused on BM gene expression kinetics and showed that it recapitulates many aspects of development. As such, the re-expression of the embryonic col14a1a gene indicated that col14a1a is part of the regeneration-specific program. We showed that laminins and col14a1a genes display similar kinetics and that the corresponding proteins are spatially and temporally controlled during regeneration. Analysis of our CRISPR/Cas9-mediated col14a1a knockout fish showed that collagen XIV-A contributes to timely deposition of laminins. As changes in ECM organization can affect tissue mechanical properties, we analyzed the biomechanics of col14a1a-/- regenerative BM using atomic force microscopy (AFM). Our data revealed a thinner BM accompanied by a substantial increase of the stiffness when compared to controls. Further AFM 3D-reconstructions showed that BM is organized as a checkerboard made of alternation of soft and rigid regions that is compromised in mutants leading to a more compact structure. We conclude that collagen XIV-A transiently acts as a molecular spacer responsible for BM structure and biomechanics possibly by helping laminins integration within regenerative BM. |
| Databáze: | OpenAIRE |
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