Beneficial actions of esculentin-2CHa(GA30) on high sucrose-induced oxidative stress in Drosophila melanogaster
| Autor: | Adeola O. Adedara, Opeolu O. Ojo, Oluwabukola M. Farodoye, Omolola A Adesanoye, Amos O. Abolaji, Ayodele A. Falobi |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
High sucrose
medicine.medical_specialty Esculentin Sucrose Antioxidant medicine.medical_treatment media_common.quotation_subject Longevity Biology Toxicology medicine.disease_cause chemistry.chemical_compound Internal medicine Diabetes mellitus medicine Animals Sulfhydryl Compounds media_common fungi General Medicine biology.organism_classification medicine.disease Catalase Glutathione Oxidative Stress Endocrinology Drosophila melanogaster Glucose chemistry Oxidative stress Food Science Antimicrobial Cationic Peptides |
| Zdroj: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 157 |
| ISSN: | 1873-6351 |
| Popis: | Hyperglycaemia-induced oxidative stress plays a critical role in the development of diabetes and its complications. This study investigated actions of esculentin-2CHa(GA30) on high sucrose-induced oxidative stress in adult Drosophila melanogaster. Adult flies were exposed to diets containing graded concentrations of sucrose in the presence or absence of esculentin-2CHa(GA30) (5.0-10 μmol/kg diet) for 7 days. Effects of high sucrose diet and/or esculentin-2CHa(GA30) on survival and longevity of flies, and markers of oxidative stress, antioxidant status and glucose were assessed. High-sucrose diet (15-30%) and esculentin-2CHa(GA30) (5-10 μmol/kg diet) enhanced the percentage of surviving flies by 33.5%-46.2% (P 0.01) and 7.4%-26.9% (P 0.01) respectively. Concentration-dependent reduction in total thiol (19.3-51.3%, P 0.01), reduced glutathione (22.6-54.9%, P 0.05-0.01), catalase activity (36.8-57.3%, P 0.05-0.01) and elevated glucose concentration (1.8-2.9-fold, P 0.001) were observed in high sucrose-fed flies. Esculentin-2CHa(GA30) alone did not affect levels of total thiol, reduced glutathione, glucose and catalase activity. Improved survival (1.2-1.3-fold, P 0.05-0.01) and longevity (1.3-fold) were observed in flies treated with the peptide (5.0 and 7.5 μmol/kg diet). Feeding on sucrose and esculentin-2CHa(1-30) (5.0 and 7.0 μmol/kg diet) for 7 days increased total thiol (2 - 3-fold, P 0.001) and reduced glutathione (1.6-1.8-fold, P 0.05) levels. Reduced catalase activity (21.4-36.4%, P 0.01) and reduced glucose level (38.6-49.4%, P 0.01) were observed in peptide-treated flies. Esculentin-2CHa(1-30) inhibited sucrose-induced generation of hydrogen peroxide (7.5-13.7%, P 0.05) and nitric oxide (22.3-42.9%, P 0.01) in adult flies. Overall, findings from this study offered further insights into the anti-oxidative properties of esculentin-2CHa(GA30). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect