Transport Reversal during Heteroexchange: A Kinetic Study
| Autor: | Lilia Kucheryavykh, Yuriy V. Kucheryavykh, Misty J. Eaton, Vladimir I. Makarov, Mikhail Inyushin, S. N. Skatchkov, A. Rivera |
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| Rok vydání: | 2013 |
| Předmět: |
0303 health sciences
Transporter reversal Article Subject Computer science Biomedical Engineering Biophysics Kinetic scheme Ionic bonding Substrate (chemistry) Transporter Kinetic energy computer.software_genre Transmembrane protein 03 medical and health sciences 0302 clinical medicine Membrane Data mining computer 030217 neurology & neurosurgery Research Article 030304 developmental biology |
| Zdroj: | Journal of Biophysics |
| ISSN: | 1687-8019 1687-8000 |
| DOI: | 10.1155/2013/683256 |
| Popis: | It is known that secondary transporters, which utilize transmembrane ionic gradients to drive their substrates up a concentration gradient, can reverse the uptake and instead release their substrates. Unfortunately, the Michaelis-Menten kinetic scheme, which is popular in transporter studies, does not include transporter reversal, and it completely neglects the possibility of equilibrium between the substrate concentrations on both sides of the membrane. We have developed a complex two-substrate kinetic model that includes transport reversal. This model allows us to construct analytical formulas allowing the calculation of a “heteroexchange” and “transacceleration” using standard Michaelis coefficients for respective substrates. This approach can help to understand how glial and other cells accumulate substrates without synthesis and are able to release such substrates and gliotransmitters. |
| Databáze: | OpenAIRE |
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