Effects of transcriptional mode on promoter substitution and tandem engineering for the production of epothilones in Myxococcus xanthus

Autor:	Xin-Jing Yue, Xiao-wen Cui, Wei-feng Hu, Zhi-feng Li, Youming Zhang, Yue-zhong Li, Zheng Zhang
Rok vydání:	2018
Předmět:	0301 basic medicine Myxococcus xanthus Epothilones Transcription Genetic Heterologous Epothilone Applied Microbiology and Biotechnology Industrial Microbiology 03 medical and health sciences Tandem repeat Gene cluster medicine Promoter Regions Genetic Gene Applied Genetics and Molecular Biotechnology Epothilone biosynthesis biology Transcription mode Chemistry Promoter General Medicine biology.organism_classification Cell biology 030104 developmental biology Multigene Family Tandem engineering Substitution Biotechnology medicine.drug
Zdroj:	Applied Microbiology and Biotechnology
ISSN:	1432-0614 0175-7598
Popis:	Promoter optimization is an economical and effective approach to overexpress heterologous genes and improve the biosynthesis of valuable products. In this study, we swapped the original promoter of the epothilone biosynthetic gene cluster in Myxococcus xanthus with two endogenous strong promoters PpilA and PgroEL1, respectively, which, however, decreased the epothilone production ability. The transcriptional abilities by the two promoters were found to be bloomed in the growth stage but markedly decreased after the growth, whereas the original promoter Pepo functioned majorly after the exponential growth stage. Tandem repeat engineering on the original promoter Pepo remarkably increased epothilone production. The tandem promoter exerted similar expressional pattern as Pepo did in M. xanthus. We demonstrated that differential transcriptional modes markedly affected the efficiency of promoters in controlling the gene expressions for the production of the secondary metabolite epothilones. Our study provides an insight into exploiting powerful promoters to produce valuable secondary metabolites, especially in host with limited known promoters. Electronic supplementary material The online version of this article (10.1007/s00253-018-9023-4) contains supplementary material, which is available to authorized users.
Databáze:	OpenAIRE
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