A second chain of human CD8 is expressed on peripheral blood lymphocytes
| Autor: | Jane R. Parnes, S D Gorman, L Shiue |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1988 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Cytoplasm Expression vector Base Sequence Immunology Cell Membrane Molecular Sequence Data Clone (cell biology) Transfection Articles Biology Blotting Northern Molecular biology Complementary DNA Immunology and Allergy Humans Amino Acid Sequence Beta (finance) Peptide sequence Gene CD8 |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | Human CD8 has been thought to consist of disulfide-linked homodimers and homomultimers of a single polypeptide chain homologous to mouse and rat CD8 alpha. In contrast, mouse and rat CD8 are composed of disulfide-linked heterodimers of alpha and beta chains. We have now isolated and sequenced cDNA clones encoding a human homologue of mouse and rat CD8 beta. One such clone was inserted into an expression vector and its encoded product was shown to be expressed on the cell surface after cotransfection into L cells with the human CD8 alpha gene. A second form of human CD8 beta cDNA encoding a protein with an altered cytoplasmic tail was similarly transfected, but its product could not be demonstrated on the cell surface. CD8 beta was further shown to be expressed on the surface of almost all CD8+ human peripheral blood T cells. These data provide the first evidence that human CD8 is a heterodimeric protein. |
| Databáze: | OpenAIRE |
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