Virosome-mediated delivery of tumor antigen to plasmacytoid dendritic cells
| Autor: | Laurence Chaperot, Juliette Angel, Mario Amacker, Jean-Paul Molens, Paulette Mezin, Rinaldo Zurbriggen, Jo ¨ el Plumas, Alexei Grichine |
|---|---|
| Přispěvatelé: | Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire recherche et développement, EFS, Département d'anatomie et cythologie pathologique, CHU Grenoble-Hôpital Michallon, Pevion Biotech Ltd, INSERM U823, équipe 9 (Immunobiologie et Immunothérapie des Cancers), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)-Laboratoire recherche et développement, This work was supported by Etablissement Français du sang (EFS) Grant 2003.26 and Institut National du Cancer – Cancéropôle 2004-05. JA receives funding from Ministère Français de la Recherche et de l'Enseignement Supérieur., Chaperot, Laurence |
| Rok vydání: | 2007 |
| Předmět: |
MESH: Neoplasm Proteins
MESH: Antigens Neoplasm CD8-Positive T-Lymphocytes Lymphocyte Activation 0302 clinical medicine Cytotoxic T cell Melanoma MESH: Orthomyxoviridae Antigen Presentation 0303 health sciences MESH: Dendritic Cells biology hemic and immune systems Orthomyxoviridae MESH: CD8-Positive T-Lymphocytes Tumor antigen Neoplasm Proteins 3. Good health Virosome Cell biology Infectious Diseases medicine.anatomical_structure 030220 oncology & carcinogenesis MESH: Immunotherapy Active [SDV.IMM]Life Sciences [q-bio]/Immunology Molecular Medicine Virosomes [SDV.IMM] Life Sciences [q-bio]/Immunology MESH: Melanoma T cell macromolecular substances 03 medical and health sciences MART-1 Antigen Antigen Antigens Neoplasm MHC class I medicine Humans MESH: Lymphocyte Activation 030304 developmental biology MESH: Humans General Veterinary General Immunology and Microbiology Public Health Environmental and Occupational Health Immunotherapy Active Dendritic Cells Dendritic cell MESH: Virosomes Virology CTL MESH: Antigen Presentation biology.protein |
| Zdroj: | Vaccine Vaccine, Elsevier, 2007, 25 (19), pp.3913-21. ⟨10.1016/j.vaccine.2007.01.101⟩ |
| ISSN: | 0264-410X |
| Popis: | International audience; Cytotoxic T lymphocytes (CTL) are crucial in viral clearance and tumor growth control. Thus the induction of CTL activity is an important aim in vaccine development. We investigate an innovative delivery system for peptide transfer to the MHC class I processing pathway of APC with the aim to trigger CTL in the context of an antitumoral response. The strategy relies on a novel antigen delivery system termed "chimeric immunopotentiating reconstituted influenza virosomes" (CIRIV) targeting plasmacytoid dendritic cells (PDC). By using virosomes containing encapsulated Melan-A peptide and a PDC line developed in our laboratory, we evaluated the response of Melan-A specific T cells. Virosomes have the capacity to bind PDC and are endocyted within vesicles in the cytosol. This endocytosis is inhibited by neuraminidase, suggesting that it is mediated by sialic acid present on cell surface. Furthermore, PDC loaded with Melan-A virosomes can induce a Melan-A specific T cell activation. Interestingly, they activate T cells with a better efficiency than PDC loaded with a free peptide and when PDC where previously activated by a TLR ligand. These results indicate that virosomes could be a suitable delivery system for tumor peptide in immunotherapy of cancer. |
| Databáze: | OpenAIRE |
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