Collagen type XI α1 facilitates head and neck squamous cell cancer growth and invasion
| Autor: | John C. Sok, Sarah Contrucci, Brian K. Trevelline, J A Lee, Sufi M. Thomas, Sumana Dasari, Radhika Joshi, Ann Marie Egloff, Jennifer R. Grandis, N Kumari, Sonali Joyce |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Cancer Research Pathology Carcinogenesis Cell Growth Process medicine.disease_cause Collagen Type XI Extracellular matrix extracellular matrix proteins 0302 clinical medicine Cell Movement Protein Isoforms RNA Small Interfering Aged 80 and over 0303 health sciences Gene knockdown Middle Aged invasion 3. Good health Oncology Head and Neck Neoplasms 030220 oncology & carcinogenesis Gene Knockdown Techniques tumour progression Carcinoma Squamous Cell Disease Progression Female cancer-associated fibroblasts Adult medicine.medical_specialty Cell Growth Processes Biology head and neck squamous cell carcinoma 03 medical and health sciences collagen Type XI α1 Cell Line Tumor medicine Humans Neoplasm Invasiveness RNA Messenger Molecular Diagnostics 030304 developmental biology Aged Messenger RNA Cell growth Squamous Cell Carcinoma of Head and Neck medicine.disease Head and neck squamous-cell carcinoma stomatognathic diseases Cell culture |
| Zdroj: | Grandis, Jennifer; Sok, JC; Lee, JA; Dasari, S; Joyce, S; Contrucci, SC; et al.(2013). Collagen type XI α1 facilitates head and neck squamous cell cancer growth and invasion. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/0s69b4d5 British Journal of Cancer |
| Popis: | Background: Although it is well established that the extracellular matrix affects tumour progression, not much is known about the various components and their effect on head and neck squamous cell carcinoma (HNSCC) progression. Levels of collagen type XI α1 (colXIα1), a minor fibrillar collagen, have been shown to be increased in tumour compared with normal tissue in several cancers, including colorectal, breast, and non-small cell lung cancer. Currently, the functional significance of colXIα1 is not understood. Methods: We examined the expression levels of colXIα1 mRNA and elucidated the functional role of colXIα1 in HNSCC. Cell proliferation, invasion, and migration were examined with and without colXIα1 knockdown with siRNA in HNSCC cells. Results: Our data demonstrate that colXIα1 expression is increased in tumour samples compared with levels in normal adjacent tissue in 16/23 HNSCC patients. In addition, colα11 is increased in HNSCC cell lines compared with normal immortalised epithelial cells and is increased in tumour-derived fibroblasts compared with normal fibroblasts. Using an siRNA approach, we demonstrate that colXIα1 contributes to proliferation, migration, and invasion of HNSCC. Conclusion: Our cumulative findings suggest that colXIα1 contributes to HNSCC tumorigenesis and may serve as a potential therapeutic target. |
| Databáze: | OpenAIRE |
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