Retinal pigment epithelial cells are heterogeneous in their expression of MHC-II after stimulation with interferon-gamma
| Autor: | Hideya Kimura, Katia Emiko Taba, Jose Augusto Cardillo, Antonio Marcelo Barbante Casella, Stephen J. Ryan, David R. Hinton, Christine Spee |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Proliferative vitreoretinopathy Aging Cellular and Molecular Neuroscience chemistry.chemical_compound Interferon-gamma Antigen In vivo medicine Animals Humans Microscopy Immunoelectron Pigment Epithelium of Eye Aged Retina MHC class II Retinal pigment epithelium biology Histocompatibility Antigens Class II Retinal medicine.disease Molecular biology Immunohistochemistry Sensory Systems Stimulation Chemical Ophthalmology medicine.anatomical_structure chemistry Immunology biology.protein sense organs Rabbits Immunostaining |
| Zdroj: | Experimental eye research. 68(4) |
| ISSN: | 0014-4835 |
| Popis: | The pattern of interferon-gamma-induced major histocompatibility complex Class II antigen expression was evaluated on the retinal pigment epithelium. Experiments were performed in vitro using explant cultures of aged and fetal human eyes and in vivo in albino rabbits. The human explants were stimulated with 50 U ml-1 interferon-gamma for 3 days prior to immunostaining for Class II. The rabbit eyes were subretinally injected in vivo with 50 microl of interferon-gamma (500 U ml-1) and analyzed immunohistochemically 3 days later. A heterogeneous pattern of Class II expression was present in the interferon-gamma-stimulated retinal pigment epithelial cells, in both the in vivo and the in vitro experiments. In aged human eyes the percent of Class-II positive cells was higher in the periphery than in the posterior pole (macular region) after interferon-gamma stimulation (P0.01). No such difference was found in the fetal eyes. These data demonstrate that retinal pigment epithelial cells are heterogeneous in their response to interferon-gamma. The results are supportive of previous studies demonstrating the structural and proliferative heterogeneity of the retinal pigment epithelium. Together, these studies provide support for the possibility of functional retinal pigment epithelial heterogeneity. |
| Databáze: | OpenAIRE |
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