Protease-activated receptor 2 mediates eosinophil infiltration and hyperreactivity in allergic inflammation of the airway
| Autor: | Fabien Schmidlin, Patrick G. Knott, David Lewis, Paul R. Gater, Silvia Amadesi, Karim Dabbagh, Pierangelo Geppetti, Claude Bertrand, Nigel W. Bunnett, Mary E. Stevens |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Ovalbumin Immunology Tryptase Mice Transgenic Allergic inflammation Cell Line Mice Cell Movement Immunology and Allergy Medicine Animals Humans Receptor PAR-2 Pulmonary Eosinophilia Lung Sensitization Protease-activated receptor 2 Crosses Genetic Inflammation Mice Knockout medicine.diagnostic_test biology business.industry respiratory system Eosinophil Allergens Immunoglobulin E medicine.disease Mice Inbred C57BL Bronchoalveolar lavage medicine.anatomical_structure biology.protein Methacholine Female Receptors Thrombin Bronchial Hyperreactivity business Infiltration (medical) Injections Intraperitoneal medicine.drug |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 169(9) |
| ISSN: | 0022-1767 |
| Popis: | Trypsin and mast cell tryptase can signal to epithelial cells, myocytes, and nerve fibers of the respiratory tract by cleaving proteinase-activated receptor 2 (PAR2). Since tryptase inhibitors are under development to treat asthma, a precise understanding of the contribution of PAR2 to airway inflammation is required. We examined the role of PAR2 in allergic inflammation of the airway by comparing OVA-sensitized and -challenged mice lacking or overexpressing PAR2. In wild-type mice, immunoreactive PAR2 was detected in airway epithelial cells and myocytes, and intranasal administration of a PAR2 agonist stimulated macrophage infiltration into bronchoalveolar lavage fluid. OVA challenge of immunized wild-type mice stimulated infiltration of leukocytes into bronchoalveolar lavage and induced airway hyperreactivity to inhaled methacholine. Compared with wild-type animals, eosinophil infiltration was inhibited by 73% in mice lacking PAR2 and increased by 88% in mice overexpressing PAR2. Similarly, compared with wild-type animals, airway hyperreactivity to inhaled methacholine (40 μg/ml) was diminished 38% in mice lacking PAR2 and increased by 52% in mice overexpressing PAR2. PAR2 deletion also reduced IgE levels to OVA sensitization by 4-fold compared with those of wild-type animals. Thus, PAR2 contributes to the development of immunity and to allergic inflammation of the airway. Our results support the proposal that tryptase inhibitors and PAR2 antagonists may be useful therapies for inflammatory airway disease. |
| Databáze: | OpenAIRE |
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