Clustering transmembrane-agrin induces filopodia-like processes on axons and dendrites
| Autor: | Godela Bittcher, Maik Annies, Jürgen Löschinger, Elmar Porten, Stefan Wöll, Rene Ramseger, Stephan Kröger, Christian Abraham, Markus A. Rüegg |
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| Rok vydání: | 2006 |
| Předmět: |
animal structures
Dendritic spine Time Factors Neurite Cytochalasin B Growth Cones Video microscopy Chick Embryo Biology Nervous System Antibodies Cellular and Molecular Neuroscience Mice medicine Neurites Animals Agrin Pseudopodia Growth cone Cytoskeleton Molecular Biology Cells Cultured Microscopy Video Dose-Response Relationship Drug Cell Membrane Cell Differentiation Cell Biology Dendrites Cell biology medicine.anatomical_structure nervous system Animals Newborn Neuron Filopodia |
| DOI: | 10.5451/unibas-ep2748 |
| Popis: | The transmembrane form of agrin (TM-agrin) is primarily expressed in the CNS, particularly on neurites. To analyze its function, we clustered TM-agrin on neurons using anti-agrin antibodies. On axons from the chick CNS and PNS as well as on axons and dendrites from mouse hippocampal neurons anti-agrin antibodies induced the dose- and time-dependent formation of numerous filopodia-like processes. The processes appeared within minutes after antibody addition and contained a complex cytoskeleton. Formation of processes required calcium, could be inhibited by cytochalasine D, but was not influenced by staurosporine, heparin or pervanadate. Time-lapse video microscopy revealed that the processes were dynamic and extended laterally along the entire length of the neuron. The lateral processes had growth cones at their tips that initially adhered to the substrate, but subsequently collapsed and were retracted. These data provide the first evidence for a specific role of TM-agrin in shaping the cytoskeleton of neurites in the developing nervous system. |
| Databáze: | OpenAIRE |
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