The Effects of Pitavastatin on Nuclear Factor-Kappa B and ICAM-1 in Human Saphenous Vein Graft Endothelial Culture

Autor: Bülent Demir, Esra Demir, Burak Önal, Sibel Özyazgan, A.G. Akkan, Vedat Bakuy
Přispěvatelé: Tıp Fakültesi
Rok vydání: 2019
Předmět:
0301 basic medicine
lcsh:Diseases of the circulatory (Cardiovascular) system
Article Subject
Inflammation
030204 cardiovascular system & hematology
Pharmacology
Immunofluorescence
03 medical and health sciences
0302 clinical medicine
medicine
Human Umbilical Vein Endothelial Cells
Humans
Pharmacology (medical)
Pitavastatin
Cytotoxicity
Vein
Cells
Cultured
ICAM-1
medicine.diagnostic_test
Dose-Response Relationship
Drug
Cell adhesion molecule
business.industry
Tumor Necrosis Factor-alpha
lcsh:RM1-950
NF-kappa B
General Medicine
Intercellular Adhesion Molecule-1
Up-Regulation
lcsh:Therapeutics. Pharmacology
030104 developmental biology
medicine.anatomical_structure
lcsh:RC666-701
Quinolines
Tumor necrosis factor alpha
medicine.symptom
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Cardiology and Cardiovascular Medicine
business
Research Article
medicine.drug
Zdroj: Cardiovascular Therapeutics, Vol 2019 (2019)
Cardiovascular Therapeutics
ISSN: 1755-5922
Popis: Objective. To study pitavastatin’s effects on nuclear factor-kappa B (NF-κB ) and adhesion molecules in human saphenous vein graft endothelial culture indicating its pleotropic properties. Materials and Method. Low-dose (0.1 μM/L) and high-dose (1μM/L) pitavastatin calcium were administered as a frontline therapy in human saphenous endothelial cell culture, followed by induction of inflammation by TNF-α and determination of mRNA level alterations of ICAM-1 and NF-κB genes of endothelial cells using the qRT-PCR method. Additionally, immunofluorescence method was used to show the expression of NF-κB and ICAM-1. Finally, LDH levels were determined by the ELISA method to quantify cytotoxicity. Results. ICAM-1 mRNA expression in the low-dose pitavastatin+TNF-α group was significantly higher than that in the TNF-α group and significantly lower than that in the high-dose pitavastatin+TNF-α group (for all comparisons, P = 0.001). The low-dose pitavastatin+TNF-α group had a similar NF-κB mRNA expression with TNF-α and high-dose pitavastatin+TNF-α groups. Conclusion. Pitavastatin increases ICAM-1 mRNA expression in saphenous vein endothelial cells. Furthermore, the effect of pitavastatin on adhesion molecules appears independent of NF-κB. Novel studies are needed in this field.
Databáze: OpenAIRE
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