DNA condensation and its thermal stability influenced by phospholipid bilayer and divalent cations
| Autor: | Daniela Uhríková, Adrián Lengyel, Mária Klacsová, Pavol Balgavý |
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| Rok vydání: | 2010 |
| Předmět: |
Cations
Divalent Inorganic chemistry Lipid Bilayers DNA condensation Divalent chemistry.chemical_compound Colloid and Surface Chemistry Transition metal Nickel Thermal stability Static light scattering Magnesium Physical and Theoretical Chemistry Lipid bilayer Phospholipids chemistry.chemical_classification Liposome Surfaces and Interfaces General Medicine Cobalt DNA Zinc Spectrometry Fluorescence chemistry Nucleic Acid Conformation Calcium Copper Biotechnology |
| Zdroj: | Colloids and surfaces. B, Biointerfaces. 86(1) |
| ISSN: | 1873-4367 |
| Popis: | We studied the effect of divalent alkaline earth metal cations Ca 2+ , Mg 2+ and transition metals Co 2+ , Ni 2+ , Cu 2+ and Zn 2+ on DNA condensation and its protection against thermal denaturation in presence of dioleoylphosphatidylcholine liposomes (DOPC). Experimental results have shown that Ca 2+ and Mg 2+ as well as Zn 2+ mediate DNA condensation. Cu 2+ causes DNA double helix destabilization, and does not mediate binding between DNA and DOPC liposomes. Co 2+ and Ni 2+ can interact with DNA on both ways mentioned above. Static light scattering was use to follow the size of aggregates in DNA condensation process. Phospholipid bilayer and divalent cations protect condensed DNA against thermal destabilization. The highest stabilization effect was found in aggregates with Ca 2+ and Zn 2+ , whereas in presence of either Co 2+ or Ni 2+ some volume fraction of DNA is denatured. |
| Databáze: | OpenAIRE |
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