Functionalized carbon nanotubes as transporters for antisense oligodeoxynucleotides
| Autor: | Bernd Schwenzer, Silke Hampel, Giuseppe Cirillo, Anika Kaufmann, David Kunhardt |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Materials science
Cell Biomedical Engineering Transporter General Chemistry General Medicine Carbon nanotube Cycloaddition law.invention chemistry.chemical_compound Adsorption medicine.anatomical_structure Biochemistry chemistry Cell culture law medicine Biophysics General Materials Science Viability assay DNA |
| Zdroj: | J. Mater. Chem. B. 2:7000-7008 |
| ISSN: | 2050-7518 2050-750X |
| Popis: | The use of DNA-based therapeutics requires efficient delivery systems to transport the DNA to their place of action within the cell. To accomplish this, we investigated multiwalled carbon nanotubes (pristine MWCNT, p-MWCNT) functionalized with hydroxyl groups via 1,3-dipolar cycloaddition. In this way, we have obtained MWCNT-f-OH with improved stability in aqueous dispersions which is an advantageous property for their use in cellular environments. Afterwards, a carrier strand oligodeoxynucleotide (CS-ODN) was adsorbed to MWCNT-f-OH followed by hybridization with a therapeutic antisense oligodeoxynucleotide (AS-ODN). The amount of adsorbed CS-ODN, as well as the complementary AS-ODN and a non-complementary oligodeoxynucleotide (NS-ODN) as reference, was directly measured by radionuclide labeling of ODNs. We show that subsequent release of AS-ODNs and NS-ODNs was possible for MWCNT-f-OH above the melting temperature of AS-ODNs at 80 °C and under physiological conditions at different pH values at 37 °C. We also show a very low influence of p-MWCNT and MWCNT-f-OH on the cell viability of the bladder carcinoma (BCa) cell line EJ28 and that both MWCNT types were internalized by EJ28. Therefore, MWCNT-f-OH represents a promising carrier able to transport and release AS-ODNs inside cells. |
| Databáze: | OpenAIRE |
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