Isolation of monoclonal antibodies from cell containing hybridoma broth using a protein A coated adsorbent in expanded beds
| Autor: | Andrea Bader, Maria-Regina Kula, Markus Halfar, Andreas Karau, Jörg Thömmes |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Hybridomas
Chromatography biology Chemistry Elution medicine.drug_class Expanded bed adsorption Organic Chemistry Cell Antibodies Monoclonal General Medicine Monoclonal antibody Biochemistry Analytical Chemistry law.invention medicine.anatomical_structure Adsorption law biology.protein medicine Recombinant DNA Staphylococcal Protein A Protein A Effluent |
| Zdroj: | Journal of Chromatography A. 752:111-122 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(96)00504-3 |
| Popis: | A novel expanded bed adsorbent carrying a recombinant protein A ligand was used for the isolation of monoclonal antibodies from cell containing hybridoma fermentation broth. The untreated effluent from a continuous hybridoma cultivation was applied to the stable expanded adsorbent (Streamline rProteinA), which proved to have a high capacity for the MAb studied (14 mg MAb per ml of adsorbent). A clarified and highly concentrated (up to 50 fold) eluate of high purity was obtained. A scale up of the MAb purification is demonstrated from lab scale (250 mg MAb per purification cycle) to a small pilot scale (2 g MAb per cycle). Low product concentration in the broth in combination with the high capacity of the adsorbent caused long sample application cycles (10-11 h). Experimental problems arising from these long cycle times are discussed with regard to a large scale application of the method. |
| Databáze: | OpenAIRE |
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