Relief of diabetes by duodenal-jejunal bypass sleeve implantation in the high-fat diet and streptozotocin-induced diabetic rat model is associated with an increase in GLP-1 levels and the number of GLP-1-positive cells
| Autor: | Zhining Fan, Xiangyang Shi, Xiang Wang, Xueming Tan, Limei Ma, Guanyin Xiong, Ying Zhang, Xiuhua Zhang, Jinquan Shuang, Shiqing Du, Yongsong Gu, Zhonghua Jiang, Jing Huang |
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| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
medicine.medical_specialty streptozotocin Immunology and Microbiology (miscellaneous) Internal medicine Diabetes mellitus medicine Glycemic duodenal-jejunal bypass sleeve business.industry rat model Sham surgery General Medicine Articles medicine.disease Streptozotocin Glucagon-like peptide-1 Small intestine medicine.anatomical_structure Endocrinology glucagon-like peptide-1 diabetes mellitus Duodenum business Intestinal L Cells medicine.drug |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-0981 |
| Popis: | A recently invented duodenal-jejunal bypass sleeve (DJBS) implanted in the duodenum and proximal jejunum has exhibited good glycemic control in diabetes mellitus. However, the specific mechanism by which DJBS placement induces the remission of diabetes is not well known. Previous studies have indicated that changes in the pattern of gut hormone secretion may play a role. The aim of the present study was to explore the role of intestinal L cells and the production of glucagon-like peptide-1 (GLP-1) by these cells in DJBS implantation-induced glycemic control in diabetic rats. A DJBS was placed in the proximal small intestine of rats with diabetes induced by a high-fat diet and low-dose streptozotocin (STZ), and the effects of the DJBS on the remission of diabetes and the GLP-1 levels of plasma and intestinal tissues were investigated 12 weeks after DJBS placement. The number of intestinal GLP-1 positive cells was also counted. When the DJBS had been in place for 12 weeks, the plasma glucose level of the DJBS-implanted rats decreased significantly from 23.33±1.56 mmol/l prior to surgery to 7.70±0.84 mmol/l and the diabetes mellitus was relieved completely; however, diabetic control rats and diabetic rats subjected to sham surgery did not show any improvement. Parallel with the remission of diabetes, the plasma and distal ileum GLP-1 levels of rats in the DJBS implantation group were also higher than those of rats in the diabetic control and sham surgery groups. The number of GLP-1-positive cells in the distal ileum was also higher in the DJBS implantation group than in the diabetic control and sham surgery groups (31.0±2.6 vs. 23.5±4.4 vs. 23.0±3.2 respectively; P |
| Databáze: | OpenAIRE |
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