The pioneer transcription factors Foxa1 and Foxa2 regulate alternative RNA splicing during thymocyte positive selection
| Autor: | Ching-In Lau, Susan Ross, Anisha Solanki, Jasmine Rowell, Masahiro Ono, Diana C. Yánez, Tessa Crompton |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Hepatocyte Nuclear Factor 3-alpha RNA splicing Mouse T-Lymphocytes Mice Transgenic Thymus Gland Foxa1 Foxa2 T-cell development PTPRC 03 medical and health sciences Exon Mice 0302 clinical medicine Conditional gene knockout Animals Molecular Biology Gene Transcription factor Thymocytes biology Alternative splicing Exons Cell biology Thymus Mice Inbred C57BL Positive selection Thymocyte Alternative Splicing 030104 developmental biology biology.protein Hepatocyte Nuclear Factor 3-beta RNA Splicing Factors 030215 immunology Developmental Biology Research Article |
| Zdroj: | Development (Cambridge, England) article-version (VoR) Version of Record |
| ISSN: | 1477-9129 |
| Popis: | During positive selection at the transition from CD4+CD8+ double-positive (DP) to single-positive (SP) thymocyte, TCR signalling results in appropriate MHC restriction and signals for survival and progression. We show that the pioneer transcription factors Foxa1 and Foxa2 are required to regulate RNA splicing during positive selection of mouse T cells and that Foxa1 and Foxa2 have overlapping/compensatory roles. Conditional deletion of both Foxa1 and Foxa2 from DP thymocytes reduced positive selection and development of CD4SP, CD8SP and peripheral naïve CD4+ T cells. Foxa1 and Foxa2 regulated the expression of many genes encoding splicing factors and regulators, including Mbnl1, H1f0, Sf3b1, Hnrnpa1, Rnpc3, Prpf4b, Prpf40b and Snrpd3. Within the positively selecting CD69+DP cells, alternative RNA splicing was dysregulated in the double Foxa1/Foxa2 conditional knockout, leading to >850 differentially used exons. Many genes important for this stage of T-cell development (Ikzf1-3, Ptprc, Stat5a, Stat5b, Cd28, Tcf7) and splicing factors (Hnrnpab, Hnrnpa2b1, Hnrnpu, Hnrnpul1, Prpf8) showed multiple differentially used exons. Thus, Foxa1 and Foxa2 are required during positive selection to regulate alternative splicing of genes essential for T-cell development, and, by also regulating splicing of splicing factors, they exert widespread control of alternative splicing. Summary: The pioneer transcription factors Foxa1 and Foxa2 are required for positive selection during T-cell development in the thymus for regulation of RNA splicing. |
| Databáze: | OpenAIRE |
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