Antifungal Properties of Nerolidol-Containing Liposomes in Association with Fluconazole

Autor: Allyson Pontes Pinheiro, Jaime Ribeiro-Filho, Taís Gusmão da Silva, José Maria Barbosa Filho, Rosilaine de Lima Honorato, Débora Lima Sales, Janaína Esmeraldo Rocha, José Geraldo de Alencar Santos Júnior, Camila Fonseca Bezerra, Thiago Sampaio de Freitas, Antonia Thassya Lucas dos Santos, Josefa Carolaine Pereira da Silva, Teresinha Gonçalves da Silva, Maria Flaviana Bezerra Morais-Braga, Gabriela Ribeiro de Sousa, Antonio Linkoln Alves Borges Leal, Thiago Adler Tavares Vieira, Henrique Douglas Melo Coutinho
Rok vydání: 2020
Předmět:
Zdroj: Membranes
Membranes, Vol 10, Iss 194, p 194 (2020)
Volume 10
Issue 9
ISSN: 2077-0375
Popis: (1) Background: Infections by Candida species represent a serious threat to the health of immunocompromised individuals. Evidence has indicated that nerolidol has significant antifungal properties. Nonetheless, its use is restricted due to a low water solubility and high photosensitivity. The incorporation into liposomes may represent an efficient alternative to improve the physicochemical and biopharmaceutical properties of this compound. The present study aimed to characterize the antifungal properties of liposomal nerolidol, alone or in combination with fluconazole. Of note, this is the first study reporting the antifungal activity of liposomal nerolidol and its potentiating effect in association with fluconazole. (2) Methods: The Inhibitory Concentration 50%-IC50 and minimum fungicide concentrations (MFC) of the substances against Candida albicans (CA), Candida tropicalis (CT), and Candida krusei (CK) were established by subculture in a solid medium. To evaluate the antifungal-enhancing effect, the MFC of fluconazole was determined in the presence or absence of subinhibitory concentrations of nerolidol (free or liposomal). The analysis of fungal dimorphism was performed through optical microscopy and the characterization of liposomes was carried out considering the vesicular size, polydispersion index, and zeta medium potential, in addition to a scanning electron microscopy analysis. (3) Results: The physicochemical characterization revealed that liposomes were obtained as homogenous populations of spherical vesicles. The data obtained in the present study indicate that nerolidol acts as an antifungal agent against Candida albicans and Candida tropicalis, in addition to potentiating (only in the liposomal form) the effect of fluconazole. However, the compound had little inhibitory effect on fungal dimorphism. (4) Conclusions: The incorporation of nerolidol into liposomes improved its antifungal-modulating properties.
Databáze: OpenAIRE
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