Human polyomavirus and human papillomavirus prevalence and viral load in non-malignant tonsillar tissue and tonsillar carcinoma
| Autor: | Martin Hellmich, Steffi Silling, Ulrike Wieland, Stephan Herberhold, Marcus Panning, Baki Akgül, Eva Bartok |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Adult Male Tonsillar Carcinoma Pathology medicine.medical_specialty Biopsy Immunology Palatine Tonsil Tonsillar Neoplasms Merkel cell polyomavirus 03 medical and health sciences Young Adult 0302 clinical medicine Risk Factors medicine Carcinoma Prevalence Immunology and Allergy Tonsil cancer Humans Papillomaviridae Aged Retrospective Studies Polyomavirus Infections Hyperplasia biology business.industry Papillomavirus Infections Cancer General Medicine Middle Aged Viral Load medicine.disease biology.organism_classification Virology Tumor Virus Infections 030104 developmental biology medicine.anatomical_structure Tonsillar Squamous Cell Carcinoma 030220 oncology & carcinogenesis Tonsil DNA Viral Female business Polyomavirus Viral load |
| Zdroj: | Medical microbiology and immunology. 206(2) |
| ISSN: | 1432-1831 |
| Popis: | Human papillomaviruses (HPVs) are an acknowledged cause of a subset of oropharyngeal cancers, especially of tonsillar cancer. Similar to HPV, some human polyomaviruses (HPyVs), such as Merkel cell polyomavirus (MCPyV), have an oncogenic potential. Recently, several novel HPyVs have been discovered. The aim of our study was to determine viral DNA prevalence and viral DNA load of 13 different HPyVs in benign and malignant tonsillar tissue and to compare the data with those found for HPV. A total of 78 biopsies of palatine tonsils with a histologic diagnosis of non-malignant disease (chronic tonsillitis, tonsillar hyperplasia, n = 40) or tonsillar squamous cell carcinoma (n = 38) were included in the study. HPyV DNA prevalence and viral load were determined by virus-specific quantitative real-time PCRs. JCPyV (1/40, 2.5%) and WUPyV (3/40, 7.5%) were only found in non-malignant tonsillar tissue. HPyV7 and HPyV10 were only detected in one (2.6%) and seven (18.4%) of the 38 cancer biopsies, respectively. Both MCPyV (8/38, 21.1 vs. 4/40, 10.0%) and HPyV6 (2/38, 5.3 vs. 1/40, 2.5%) were found more frequently in cancer samples than in non-malignant tissue, but the differences were not significant. BKPyV, KIPyV, TSPyV, HPyV9, STLPyV, HPyV12 and NJPyV were not discovered in any of the samples. HPyV loads found in HPyV DNA-positive biopsies were very low with no difference between non-malignant and malignant samples (median load |
| Databáze: | OpenAIRE |
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