Photobleaching of the 'Raman Spectroscopic Signature of Life' and Mitochondrial Activity in Rho− Budding Yeast Cells
| Autor: | Chikao Onogi, Hiro-o Hamaguchi |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Photobleaching
Strain (chemistry) biology Chemistry Saccharomyces cerevisiae Saccharomyces bayanus Mitochondrion Spectrum Analysis Raman biology.organism_classification Laser Mitochondria Surfaces Coatings and Films law.invention Polyploidy Saccharomyces symbols.namesake Crystallography law Materials Chemistry symbols Irradiation Physical and Theoretical Chemistry Raman spectroscopy |
| Zdroj: | The Journal of Physical Chemistry B. 113:10942-10945 |
| ISSN: | 1520-5207 1520-6106 |
| DOI: | 10.1021/jp903478r |
| Popis: | Raman spectra of mitochondria in single living budding yeast (zygote of Saccharomyces cerevisiae and Saccharomyces bayanus) cells have been measured and compared for two types of tetraploid strains, the wild-type strain and the rho- strain. The "Raman spectroscopic signature of life", a strong Raman band at 1602 cm(-1) that sharply reflects the mitochondrial activity, has been found in both of the two strains with similar intensities. This signature showed photobleaching under irradiation of the 632.8 nm line of a He-Ne laser, only for the rho- strain. The photobleaching recovered by keeping the cell in the dark for two hours without laser irradiation. These findings indicate that the molecular species that gives rise to the 1602 cm(-1) band is an intermediate formed and accumulated during the metabolic cycle and that it absorbs at 632.8 nm to undergo photodecomposition. We also found a number of weak Raman bands in the 400-1200 cm(-1) region that show the same temporal behaviors as that of the 1602 cm(-1) band by an SVD analysis of time-resolved Raman spectra. Several new pieces of information on the origin of the "Raman spectroscopic signature of life" have thus been obtained. |
| Databáze: | OpenAIRE |
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