Production of Minor Ginsenosides C-K and C-Y from Naturally Occurring Major Ginsenosides Using Crude β-Glucosidase Preparation from Submerged Culture of Fomitella fraxinea
| Autor: | Myung-Kon Kim, Won-Jae Lee, Sung-Ryong Ko, Jitendra Upadhyaya, Dae-Woon Kim, Yoseph Asmelash Gebru, Young-Hoi Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Liquid culture
compound-K Pharmaceutical Science High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound QD241-441 Biotransformation Drug Discovery Compound K mushroom Physical and Theoretical Chemistry β glucosidase Fomitella fraxinea Chromatography biology Chemistry major ginsenosides Fomitella Organic Chemistry biology.organism_classification enzyme preparation Chemistry (miscellaneous) Ginsenoside compound-Y Molecular Medicine Protopanaxadiol biotransformation |
| Zdroj: | Molecules Volume 26 Issue 16 Molecules, Vol 26, Iss 4820, p 4820 (2021) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules26164820 |
| Popis: | Minor ginsenosides, such as compounds (C)-K and C-Y, possess relatively better bioactivity than those of naturally occurring major ginsenosides. Therefore, this study focused on the biotransformation of major ginsenosides into minor ginsenosides using crude β-glucosidase preparation isolated from submerged liquid culture of Fomitella fraxinea (FFEP). FFEP was prepared by ammonium sulfate (30–80%) precipitation from submerged culture of F. fraxinea. FFEP was used to prepare minor ginsenosides from protopanaxadiol (PPD)-type ginsenoside (PPDG-F) or total ginsenoside fraction (TG-F). In addition, biotransformation of major ginsenosides into minor ginsenosides as affected by reaction time and pH were investigated by TLC and HPLC analyses, and the metabolites were also identified by UPLC/negative-ESI-Q-TOF-MS analysis. FFEP biotransformed ginsenosides Rb1 and Rc into C-K via the following pathways: Rd → F2 → C-K for Rb1 and both Rd → F2→ C-K and C-Mc1 → C-Mc → C-K for Rc, respectively, while C-Y is formed from Rb2 via C-O. FFEP can be applied to produce minor ginsenosides C-K and C-Y from PPDG-F or TG-F. To the best of our knowledge, this study is the first to report the production of C-K and C-Y from major ginsenosides by basidiomycete F. fraxinea. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|