Profiling and Identification of Cerebrospinal Fluid Proteins in a Rat EAE Model of Multiple Sclerosis
| Autor: | Rainer Bischoff, Christoph Stingl, Theo M. Luider, Hans van Aken, Ernst Suidgeest, Therese Rosenling, Rogier Q. Hintzen, Peter Horvatovich, Frank Suits, Marcel P. Stoop, Christin Christin, Amos Attali, Tinka Tuinstra |
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| Přispěvatelé: | Analytical Biochemistry, Medicinal Chemistry and Bioanalysis (MCB), Neurology |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
EXPRESSION Encephalomyelitis Autoimmune Experimental GPX3 Proteome PROTEOMICS ANALYSIS Encephalomyelitis experimental autoimmune encephalomyelitis Blood–brain barrier multiple sclerosis EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS DISEASE-ACTIVITY Biochemistry Mass Spectrometry cerebrospinal fluid Cerebrospinal fluid proteomics medicine Animals Paralysis OXIDATIVE STRESS VITAMIN-D biology Chemistry BLOOD-BRAIN-BARRIER Multiple sclerosis Experimental autoimmune encephalomyelitis Body Weight Cerebrospinal Fluid Proteins QTOF peptide profiling General Chemistry MASS-SPECTROMETRY medicine.disease Myelin basic protein Rats Disease Models Animal medicine.anatomical_structure Rats Inbred Lew Immunology biology.protein Orbitrap peptide profiling SPINAL-CORD Chromatography Liquid |
| Zdroj: | Journal of Proteome Research, 11(4), 2048-2060. NLM (Medline) Journal of Proteome Research, 11(4), 2048-2060. American Chemical Society |
| ISSN: | 1535-3907 1535-3893 |
| Popis: | The experimental autoimmune encephalomyelitis (EAR) model resembles certain aspects of multiple sclerosis (MScl), with common features such as motor dysfunction, axonal degradation, and infiltration of T-cells. We studied the cerebrospinal fluid (CSF) proteome in the EAE rat model to identify proteomic changes relevant for MSd disease pathology. EAE was induced in male Lewis rats by injection of myelin basic protein (MBP) together with complete Freund's adjuvant (CFA). An inflammatory control group was injected with CFA alone, and a nontreated group served as healthy control. CSF was collected at day 10 and 14 after immunization and analyzed by bottom-up proteomics on Orbitrap LC-MS and QTOF LC-MS platforms in two independent laboratories. By combining results, 44 proteins were discovered to be significantly increased in EAE animals compared to both control groups, 25 of which have not been mentioned in relation to the EAE model before. Lysozyme Cl, fetuin B, T-kininogen, serum paraoxonase/arylesterase 1, glutathione peroxidase 3, complement C3, and afamin are among the proteins significantly elevated in this rat EAE model. Two proteins, afamin and complement C3, were validated in an independent sample set using quantitative selected reaction monitoring mass spectrometry. The molecular weights of the identified differentially abundant proteins indicated an increased transport across the blood brain barrier (BBB) at the peak of the disease, caused by an increase in BBB permeability. |
| Databáze: | OpenAIRE |
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