Interferon-γ suppresses S100A4 transcription independently of apoptosis or cell cycle arrest
| Autor: | Eivind Hovig, Kristin Andersen, K B Pedersen, Øystein Fodstad, Birgitte Smith-Sørensen, Ola Myklebost, Gunhild Mari Mælandsmo |
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| Rok vydání: | 2003 |
| Předmět: |
Cancer Research
Programmed cell death Cell cycle checkpoint Transcription Genetic RNA Stability Apoptosis Breast Neoplasms colon carcinoma Biology Interferon-gamma Downregulation and upregulation osteosarcoma Tumor Cells Cultured Humans Experimental Therapeutics S100 Calcium-Binding Protein A4 mammary carcinoma Cell Line Transformed Oligonucleotide Array Sequence Analysis Regulation of gene expression Cell Cycle S100 Proteins Cell cycle Gene Expression Regulation Oncology Colonic Neoplasms Immunology Cancer cell Cancer research Jak-STAT1 signalling Signal transduction microarray Signal Transduction |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1038/sj.bjc.6600998 |
| Popis: | The S100A4 protein has been associated with increased metastatic capacity of cancer cells, and recent studies have suggested a correlation between the expression level of S100A4 and the prognostic outcome for patients with various types of cancer. The knowledge about the mechanisms underlying the metastasis-promoting effects is still limited, and the aim of the present study was to elucidate signal transduction pathways involved in the regulation of S100A4. After treatment of human carcinoma cells with interferon-gamma (IFN-gamma), we observed downregulation of S100A4 both at mRNA and protein levels. The effect was not dependent on IFN-gamma-induced apoptosis or IFN-gamma-mediated cell cycle arrest. Moreover, IFN-gamma-mediated decrease in mRNA stability could not account for the observed decrease in S100A4 transcript level. Finally, microarray analysis suggests ISGF3G, ETV5, ZNF133 and CEBPG as possible candidate genes involved in IFN-gamma-mediated repression of S100A4. |
| Databáze: | OpenAIRE |
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