Regulation of tRNA synthesis by the general transcription factors of RNA polymerase III - TFIIIB and TFIIIC, and by the MAF1 protein
| Autor: | Damian Graczyk, Małgorzata Cieśla, Magdalena Boguta |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins Transcription Genetic Biophysics Saccharomyces cerevisiae Biochemistry RNA polymerase III Mice 03 medical and health sciences chemistry.chemical_compound RNA Transfer Transcription Factor TFIIIB Transcription Factors TFIII Structural Biology Transcription (biology) Gene Expression Regulation Fungal Genetics Animals Humans Initiation factor Phosphorylation Molecular Biology Gene Mammals General transcription factor Chemistry RNA Polymerase III Cell biology 030104 developmental biology Gene Expression Regulation Transfer RNA Maf Transcription Factors Human genome RNA Polymerase II Protein Processing Post-Translational DNA |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms. 1861:320-329 |
| ISSN: | 1874-9399 |
| Popis: | The synthesis of transfer RNA (tRNA) is directed by RNA polymerase III (Pol III) specialized in high-level transcription of short DNA templates. Pol III recruitment to tRNA genes is controlled by two general initiation factors, TFIIIB and TFIIIC. They are multi-protein complexes regulated at the level of expression of individual subunits, as well as through phosphorylation and interaction with partner proteins. Here, we describe particular aspects of TFIIIB and TFIIIC control in yeast and human cells. Under stress conditions, tRNA synthesis is negatively regulated by the MAF1 protein, which interacts directly with Pol III. Sequence and function of MAF1 are conserved among eukaryotic organisms from yeast to humans. MAF1 is a phosphoprotein which mediates diverse regulatory signals to Pol III. Interestingly, there is a subset of housekeeping tRNA genes, both in the yeast and human genome, which are less sensitive to MAF1-dependent repression. The possible mechanisms responsible for this differential regulation of tRNA synthesis by MAF1 are discussed. |
| Databáze: | OpenAIRE |
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