Israeli Rousettus aegyptiacus Pox Virus (IsrRAPXV) Infection in Juvenile Egyptian Fruit Bat (Rousettus aegyptiacus): Clinical Findings and Molecular Detection
Autor: | Nir Edery, Dan David, Sharon Karniely, Asaf Sol, Ariela Rosenzweig, Irit Davidson |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
tongue lesions Sequence analysis DNA polymerase viruses 030231 tropical medicine lcsh:QR1-502 skin lesions Virus lcsh:Microbiology Lesion 03 medical and health sciences 0302 clinical medicine Virology medicine Juvenile Gene IsrRAPXV biology virus diseases biology.organism_classification 030104 developmental biology Infectious Diseases Real-time polymerase chain reaction Egyptian fruit bat (Rousettus aegyptiacus) poxvirus biology.protein medicine.symptom Rousettus |
Zdroj: | Viruses, Vol 13, Iss 407, p 407 (2021) |
ISSN: | 1999-4915 |
Popis: | During 2019, five carcasses of juvenile Egyptian fruit bats (Rousettus aegyptiacus) were submitted to the Kimron Veterinary Institute. These bats exhibited typical poxvirus like lesion plaques of different sizes on the skin, abdomen and the ventral side of the wings. Clinical and histopathological findings suggested a poxvirus infection. Infectious virus was isolated from skin swabs, skin tissue and tongue of the dead bats and was further confirmed to be a Poxvirus by molecular diagnosis using PCR with pan-chordopoxviruses primers. All the dead bats were found positive for two Poxvirus genes encoding a metalloproteinase and DNA dependent DNA polymerase. In this study, a novel real time quantitative PCR (qPCR) assay was established to further confirmed the presence of specific poxvirus viral DNA in all pathologically tested tissues. Moreover, according to sequence analysis, the virus was found to be highly similar to the recently discovered Israeli Rousettus aegyptiacus Pox Virus (IsrRAPXV). |
Databáze: | OpenAIRE |
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