Detection and Quantitation of Selected Food Allergens by Liquid Chromatography with Tandem Mass Spectrometry: First Action 2017.17
| Autor: | Alex Liu, Sharon L Brunelle, Mark Cafazzo, Jianru Stahl-Zeng, Hua-Fen Liu, Lee Sun New, Andre Schreiber |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.disease_cause
Tandem mass spectrometry 01 natural sciences Analytical Chemistry Allergen food Tandem Mass Spectrometry medicine Humans Environmental Chemistry Food allergens Mathematics Pharmacology Wine Chromatography 010405 organic chemistry digestive oral and skin physiology 010401 analytical chemistry Selected reaction monitoring food and beverages Breakfast cereal Repeatability Allergens food.food Food Analysis 0104 chemical sciences Agronomy and Crop Science Food Hypersensitivity Chromatography Liquid Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 103:570-583 |
| ISSN: | 1944-7922 1060-3271 |
| Popis: | Background: In response to a need for accurate and reliable methods for food allergen regulatory compliance, a method for the detection and quantitation of whole egg, whole milk, peanut, and hazelnut in eight food matrices was developed and evaluated in a single-laboratory validation. The matrices include cookies, cookie dough, bread, breakfast cereal, salad dressing, ice cream, and red wine. Objective: The method was compared with Standard Method Performance Requirements (SMPR) 2016.002 established by the AOAC Stakeholder Panel on Strategic Food Analytical Methods. Methods: The method involves tryptic digestion of allergen proteins in food matrices incurred or spiked with allergen standards [reference materials (RMs), Standard RMs (SRMs), or in-house prepared standard] and uses labeled peptide internal standards. LC-tandem MS analysis of the signature tryptic peptides of the four allergens is performed using multiple reaction monitoring. Results: For 10 allergen/matrix combinations, the method demonstrated adequate sensitivity with a minimum quantitation limit of 3 mg/kg for whole egg and 10 mg/kg for milk, peanut, and hazelnut allergens. Repeatability precision across 3 days of analyses was |
| Databáze: | OpenAIRE |
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