A novel natural tracer method to measure complex carbohydrate metabolism
| Autor: | Michele Schiavon, Xuan Mai Petterson, Michael Q. Slama, Ananda Basu, Rita Basu, Rickey E. Carter, Chiara Dalla Man, Ling Hinshaw, Claudio Cobelli |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male medicine.medical_specialty complex carbohydrates endogenous glucose production glucose metabolism Isotope dilution method Physiology Accurate estimation Endocrinology Diabetes and Metabolism Carbohydrate metabolism Mixed meal Young Adult Complex carbohydrate Polysaccharides Insulin-Secreting Cells Physiology (medical) TRACER Internal medicine Dietary Carbohydrates medicine Humans Meals Sorghum Carbon Isotopes Chromatography Chemistry Oryza Metabolism Carbohydrate Postprandial Period Healthy Volunteers Glucose Endocrinology Carbohydrate Metabolism Female Insulin Resistance Radiopharmaceuticals Algorithms Research Article |
| Zdroj: | Am J Physiol Endocrinol Metab |
| Popis: | While the triple tracer isotope dilution method has enabled accurate estimation of carbohydrate turnover after a mixed meal, use of the simple carbohydrate glucose as the carbohydrate source limits its translational applicability to everyday meals that typically contain complex carbohydrates. Hence, utilizing the natural enrichment of [13C]polysaccharide in commercially available grains, we devised a novel tracer method to measure postprandial complex carbohydrate turnover and indices of insulin action and β-cell function and compared the parameters to those obtained after a simple carbohydrate containing mixed meal. We studied healthy volunteers after either rice ( n = 8) or sorghum ( n = 8) and glucose ( n = 16) containing mixed meals and modified the triple tracer technique to calculate carbohydrate turnover. All meals were matched for calories and macronutrient composition. Rates of meal glucose appearance (2,658 ± 736 vs. 4,487 ± 909 μM·kg−1·2 h−1), endogenous glucose production (−835 ± 283 vs. −1,123 ± 323 μM·kg−1·2 h−1) and glucose disappearance (1,829 ± 807 vs. 3,606 ± 839 μM·kg−1·2 h−1) differed ( P < 0.01) between complex and simple carbohydrate containing meals, respectively. Interestingly, there were significant increase in indices of insulin sensitivity (32.5 ± 3.5 vs. 25.6 ± 3.2 10−5(dl·kg−1·min−2)/pM, P = 0.006) and β-cell responsivity (disposition index: 1,817 ± 234 vs. 1,236 ± 159 10−14(dl·kg−1·min−2)/pM, P < 0.005) with complex than simple carbohydrate meals. We present a novel triple tracer approach to estimate postprandial turnover of complex carbohydrate containing mixed meals. We also report higher insulin sensitivity and β-cell responsivity with complex than with simple carbohydrates in mixed meals of identical calorie and macronutrient compositions in healthy adults. |
| Databáze: | OpenAIRE |
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