Salmonella-Mediated Inflammation Eliminates Competitors for Fructose-Asparagine in the Gut
| Autor: | Karl K. Weitz, Mikayla A. Borton, Ryan S. Renslow, Vicki H. Wysocki, Anice Sabag-Daigle, Stephen R. Lindemann, Kelly C. Wrighton, Thomas O. Metz, Venkat Gopalan, Jikang Wu, Siwei Wei, Prosper N. Boyaka, Brooke L. Deatherage Kaiser, Carrie D. Nicora, Linnea F. M. Kop, Young-Mo Kim, Joshua N. Adkins, Blake E Szkoda, Brian M. M. Ahmer |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Salmonella Immunology Inflammation Fructose medicine.disease_cause Microbiology 03 medical and health sciences chemistry.chemical_compound Clostridium Dry weight medicine Animals Pathogen Salmonella Infections Animal biology Salmonella enterica biology.organism_classification Molecular Pathogenesis Pathogenicity island Gastrointestinal Microbiome 030104 developmental biology Infectious Diseases chemistry Fructose-asparagine Parasitology Asparagine medicine.symptom |
| Zdroj: | Infection and Immunity. 86 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | Salmonella enterica elicits intestinal inflammation to gain access to nutrients. One of these nutrients is fructose-asparagine (F-Asn). The availability of F-Asn to Salmonella during infection is dependent upon Salmonella pathogenicity islands 1 and 2, which in turn are required to provoke inflammation. Here, we determined that F-Asn is present in mouse chow at approximately 400 pmol/mg (dry weight). F-Asn is also present in the intestinal tract of germfree mice at 2,700 pmol/mg (dry weight) and in the intestinal tract of conventional mice at 9 to 28 pmol/mg. These findings suggest that the mouse intestinal microbiota consumes F-Asn. We utilized heavy-labeled precursors of F-Asn to monitor its formation in the intestine, in the presence or absence of inflammation, and none was observed. Finally, we determined that some members of the class Clostridia encode F-Asn utilization pathways and that they are eliminated from highly inflamed Salmonella -infected mice. Collectively, our studies identify the source of F-Asn as the diet and that Salmonella -mediated inflammation is required to eliminate competitors and allow the pathogen nearly exclusive access to this nutrient. |
| Databáze: | OpenAIRE |
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