Cessation of cyclic stretch induces atrophy of C2C12 myotubes

Autor: David S. Criswell, Vitor A. Lira, Elizabeth H. Zeanah, Quinlyn A. Soltow
Rok vydání: 2013
Předmět:
Cytoplasm
Time Factors
Muscle Fibers
Skeletal
Nitric Oxide Synthase Type II
Nitric Oxide Synthase Type I
Biochemistry
chemistry.chemical_compound
Mice
Myosin
Myocyte
Phosphorylation
biology
Chemistry
Myogenesis
Nonmuscle Myosin Type IIA
Forkhead Box Protein O3
Calpain
Cell Differentiation
Forkhead Transcription Factors
Immunohistochemistry
Biomechanical Phenomena
Muscular Atrophy
medicine.anatomical_structure
NG-Nitroarginine Methyl Ester
Muscle Tonus
Signal Transduction
medicine.medical_specialty
Biophysics
Nitric Oxide
Nitric oxide
Atrophy
Internal medicine
Cell Line
Tumor
medicine
Animals
Molecular Biology
Protein kinase B
Cell Size
Myosin Heavy Chains
Cell Membrane
Skeletal muscle
Cell Biology
medicine.disease
Enzyme Activation
Endocrinology
Proteolysis
biology.protein
Stress
Mechanical
Proto-Oncogene Proteins c-akt
Zdroj: Biochemical and biophysical research communications. 434(2)
ISSN: 1090-2104
Popis: Cyclic stretch of differentiated myotubes mimics the loading pattern of mature skeletal muscle. We tested a cell culture model of disuse atrophy by the cessation of repetitive bouts of cyclic stretch in differentiated C2C12 myotubes. Myotubes were subjected to cyclic strain (12%, 0.7 Hz, 1 h/d) on collagen-I-coated Bioflex plates using a computer-controlled vacuum stretch apparatus (Flexcell Int.) for 2 (2dSTR) or 5 (5dSTR) consecutive days. Control cultures were maintained in the Bioflex plates without cyclic stretch for 2d or 5d. Additionally, some cultures were stretched for 2 d followed by cessation of stretch for 3d (2dSTR3dCES). Cyclic stretching (5dSTR) increased myotube diameter and overall myotube area by ~2-fold (P
Databáze: OpenAIRE
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