Cessation of cyclic stretch induces atrophy of C2C12 myotubes
Autor: | David S. Criswell, Vitor A. Lira, Elizabeth H. Zeanah, Quinlyn A. Soltow |
---|---|
Rok vydání: | 2013 |
Předmět: |
Cytoplasm
Time Factors Muscle Fibers Skeletal Nitric Oxide Synthase Type II Nitric Oxide Synthase Type I Biochemistry chemistry.chemical_compound Mice Myosin Myocyte Phosphorylation biology Chemistry Myogenesis Nonmuscle Myosin Type IIA Forkhead Box Protein O3 Calpain Cell Differentiation Forkhead Transcription Factors Immunohistochemistry Biomechanical Phenomena Muscular Atrophy medicine.anatomical_structure NG-Nitroarginine Methyl Ester Muscle Tonus Signal Transduction medicine.medical_specialty Biophysics Nitric Oxide Nitric oxide Atrophy Internal medicine Cell Line Tumor medicine Animals Molecular Biology Protein kinase B Cell Size Myosin Heavy Chains Cell Membrane Skeletal muscle Cell Biology medicine.disease Enzyme Activation Endocrinology Proteolysis biology.protein Stress Mechanical Proto-Oncogene Proteins c-akt |
Zdroj: | Biochemical and biophysical research communications. 434(2) |
ISSN: | 1090-2104 |
Popis: | Cyclic stretch of differentiated myotubes mimics the loading pattern of mature skeletal muscle. We tested a cell culture model of disuse atrophy by the cessation of repetitive bouts of cyclic stretch in differentiated C2C12 myotubes. Myotubes were subjected to cyclic strain (12%, 0.7 Hz, 1 h/d) on collagen-I-coated Bioflex plates using a computer-controlled vacuum stretch apparatus (Flexcell Int.) for 2 (2dSTR) or 5 (5dSTR) consecutive days. Control cultures were maintained in the Bioflex plates without cyclic stretch for 2d or 5d. Additionally, some cultures were stretched for 2 d followed by cessation of stretch for 3d (2dSTR3dCES). Cyclic stretching (5dSTR) increased myotube diameter and overall myotube area by ~2-fold (P |
Databáze: | OpenAIRE |
Externí odkaz: |