A physical map of the chicken genome
| Autor: | Shin Leong, Debra E Scheer, Mary J Fedele, Michael N Romanov, Jason Carter, Jason Walker, Mikhail Nefedov, Martin Krzywinski, Jerry B. Dodgson, Elaine R. Mardis, Pieter J. de Jong, Martien A. M. Groenen, John Douglas Mcpherson, Asif T. Chinwalla, Jan Aerts, Kelly Mead, LaDeana W. Hillier, Colin Kremitzki, Hans H. Cheng, Jacquie Schein, Richard P. M. A. Crooijmans, Derek Albracht, Jamey Higginbotham, Dan Layman, Shiaw-Pyng Yang, Kazutoyo Osoegawa, Mandeep Sekhon, Marco Cardenas, Tina Graves, Rebecca McGrane, Hong-Bin Zhang, John W. Wallis, Wesley C. Warren, Richard K. Wilson, Jonathan Davito, Tony Gaige, Kristine M. Wylie, Nancy K Mudd |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Chromosomes
Artificial Bacterial Genetic Linkage Hybrid genome assembly Genomics integration mouse genome Computational biology Biology Animal Breeding and Genomics Genome Sequence-tagged site Contig Mapping Animals Fokkerij en Genomica Cloning Molecular QH426 Sequence Tagged Sites Genetics Multidisciplinary Contig bac clones Shotgun sequencing software Genome project sequence Physical Chromosome Mapping DNA Fingerprinting WIAS linkage Chickens Reference genome |
| Zdroj: | Nature, 432(7018), 761-764 Nature 432 (2004) 7018 |
| ISSN: | 0028-0836 1476-4687 |
| Popis: | Strategies for assembling large, complex genomes have evolved to include a combination of whole-genome shotgun sequencing and hierarchal map-assisted sequencing1, 2. Whole-genome maps of all types can aid genome assemblies, generally starting with low-resolution cytogenetic maps and ending with the highest resolution of sequence. Fingerprint clone maps are based upon complete restriction enzyme digests of clones representative of the target genome, and ultimately comprise a near-contiguous path of clones across the genome. Such clone-based maps are used to validate sequence assembly order, supply long-range linking information for assembled sequences, anchor sequences to the genetic map and provide templates for closing gaps. Fingerprint maps are also a critical resource for subsequent functional genomic studies, because they provide a redundant and ordered sampling of the genome with clones3. In an accompanying paper4 we describe the draft genome sequence of the chicken, Gallus gallus, the first species sequenced that is both a model organism and a global food source. Here we present a clone-based physical map of the chicken genome at 20-fold coverage, containing 260 contigs of overlapping clones. This map represents approximately 91% of the chicken genome and enables identification of chicken clones aligned to positions in other sequenced genomes |
| Databáze: | OpenAIRE |
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