Aging, aluminium and basal forebrain lesions modify substrate kinetics of erythrocyte membrane Na, K-ATPase in the rat
| Autor: | Ljubisav Rakic, Milena Erić Jovičić, Katica Jovanova Nešić, Svetlana Jovičić Pavlović, Miroljub Popović, Natalija Topić Popović |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty Kainic acid Sodium chemistry.chemical_element Substrate Specificity Lesion chemistry.chemical_compound Adenosine Triphosphate Chlorides Alzheimer Disease Internal medicine medicine Aluminum Chloride Animals Na Rats Wistar Na+/K+-ATPase Aluminum Compounds Injections Intraventricular Basal forebrain Kainic Acid Chemistry General Neuroscience aluminium Erythrocyte Membrane aging Age Factors Substrate (chemistry) General Medicine Alzheimer's disease Rats nucleus basalis magnocellularis rats Disease Models Animal Psychiatry and Mental health Clinical Psychology Endocrinology Membrane Cholinergic Fibers Biochemistry K-ATPase Basal Nucleus of Meynert Potassium Cholinergic Sodium-Potassium-Exchanging ATPase Geriatrics and Gerontology medicine.symptom |
| Zdroj: | Journal of Alzheimer's Disease |
| Popis: | Several studies suggested that the activity of erythrocyte Na, K-ATPase declines with aging. Here, it is postulated that alterations in the substrate kinetics of the erythrocyte membrane Na, K-ATPase could be more aggravated in conditions of brain cholinergic dysfunction seen in Alzheimer's disease than in normal aging. To test this hypothesis, we compared the Na, K-ATPase activity (Vmax/Km parameters) in aged rats with those in young rats with brain cholinergic dysfunction induced by electrolytic-, kainic acid-lesioned nucleus basalis magnocellularis (NBM) or by intracerebroventricular AlCl3 administration. In the above mentioned groups, Vmax values were significantly lower in comparison to the control animals. Furthermore, Km values were significantly higher in animals with electrolytic-induced NBM lesions, AlCl3 treated rats and aged animals. However, Km was significantly lower in kainic acid-induced NBM lesions compared to the control group. The Na, K-ATPase catalytic efficiency, estimated by the ratio Vm/Km, decreased as followed: young animals gt aged animals gt kainic acid lesion gt electrolityc lesion gt AlCl3. Our data suggest that neurodegenerative processes similar to those seen in Alzheimer's disease affect the sodium/potassium pump functionality which might be detected in peripheral blood erythrocyte membranes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|