Nanosilver cytotoxicity in rainbow trout (Oncorhynchus mykiss) erythrocytes and hepatocytes
| Autor: | Kathy C. Nguyen, Andrey Massarsky, Ren Abraham, Azam F. Tayabali, Peter Rippstein, Vance L. Trudeau, Thomas W. Moon |
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| Rok vydání: | 2014 |
| Předmět: |
Erythrocytes
Silver Physiology Health Toxicology and Mutagenesis Glutathione reductase Metal Nanoparticles Toxicology medicine.disease_cause Biochemistry Silver nanoparticle chemistry.chemical_compound medicine Animals Buthionine sulfoximine Cytotoxicity Glutathione Transferase chemistry.chemical_classification Reactive oxygen species Chemistry Cell Biology General Medicine Glutathione Oxidative Stress Glutathione Reductase Oncorhynchus mykiss Hepatocytes Rainbow trout Lipid Peroxidation Reactive Oxygen Species Oxidative stress DNA Damage |
| Zdroj: | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 159:10-21 |
| ISSN: | 1532-0456 |
| Popis: | Silver nanoparticles (AgNPs) are present in a multitude of consumer and medical products; however, the toxicity of AgNPs is not fully understood. This research aimed to elucidate the relationship between AgNP cytotoxicity and oxidative stress and damage in rainbow trout (Oncorhynchus mykiss) hepatocytes and erythrocytes in comparison to silver ions (Ag(+)). Generally the cytotoxicity of AgNPs and Ag(+) was similar, such that both silver types generated reactive oxygen species, decreased glutathione levels, and decreased activities of glutathione reductase and glutathione-S-transferase. Nonetheless, the two silver types had different cellular targets; AgNPs increased lipid peroxidation without apparent uptake into the cells whereas Ag(+) increased DNA damage. Furthermore, the toxicity of both silver types was generally decreased in cells treated with cysteine while treatment with buthionine sulfoximine increased the toxicity of both silver types. |
| Databáze: | OpenAIRE |
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