Low-dose irradiation affects the functional behavior of oral microbiota in the context of mucositis
| Autor: | Barbara Vanhoecke, Tine De Ryck, Simon Swift, Kevin De boel, Siouxsie Wiles, Tom Van de Wiele, Tom Boterberg |
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| Rok vydání: | 2015 |
| Předmět: |
Mucositis
0301 basic medicine Virulence Context (language use) General Biochemistry Genetics and Molecular Biology Microbiology 03 medical and health sciences 0302 clinical medicine medicine Animals Candida Original Research biology Candida glabrata Microbiota Mucin Klebsiella oxytoca Mouth Mucosa Biofilm Streptococcus biochemical phenomena metabolism and nutrition biology.organism_classification medicine.disease Lepidoptera Disease Models Animal 030104 developmental biology Streptococcus salivarius Biofilms Larva 030220 oncology & carcinogenesis |
| Zdroj: | Experimental Biology and Medicine. 241:60-70 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.1177/1535370215595467 |
| Popis: | The role of host–microbe interactions in the pathobiology of oral mucositis is still unclear; therefore, this study aimed to unravel the effect of irradiation on behavioral characteristics of oral microbial species in the context of mucositis. Using various experimental in vitro setups, the effects of irradiation on growth and biofilm formation of two Candida spp., Streptococcus salivarius and Klebsiella oxytoca in different culture conditions were evaluated. Irradiation did not affect growth of planktonic cells, but reduced the number of K. oxytoca cells in newly formed biofilms cultured in static conditions. Biofilm formation of K. oxytoca and Candida glabrata was affected by irradiation and depended on the culturing conditions. In the presence of mucins, these effects were lost, indicating the protective nature of mucins. Furthermore, the Galleria melonella model was used to study effects on microbial virulence. Irradiated K. oxytoca microbes were more virulent in G. melonella larvae compared to the nonirradiated ones. Our data indicate that low-dose irradiation can have an impact on functional characteristics of microbial species. Screening for pathogens like K. oxytoca in the context of mucosits could be useful to allow early detection and immediate intervention. |
| Databáze: | OpenAIRE |
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