Role of the chicken oligoadenylate synthase-like gene during in vitro Newcastle disease virus infection
| Autor: | Melissa S. Monson, Ana Paula Del Vesco, Hyun Jun Jang, Susan J. Lamont |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Small interfering RNA
animal structures Newcastle Disease viruses Newcastle disease virus Virus Replication Caspase 8 SF1-1100 chicken fibroblast cell line Virus 03 medical and health sciences Interferon medicine Animals Interferon alfa 030304 developmental biology 0303 health sciences Gene knockdown Oligoribonucleotides biology Full-Length Article DF-1 cells Adenine Nucleotides viral chicken disease 0402 animal and dairy science interferon 04 agricultural and veterinary sciences General Medicine small interfering RNA 040201 dairy & animal science Virology Animal culture Viral replication biology.protein Animal Science and Zoology Chickens Ribonuclease L medicine.drug |
| Zdroj: | Poultry Science Poultry Science, Vol 100, Iss 5, Pp 101067-(2021) |
| ISSN: | 0032-5791 |
| DOI: | 10.1016/j.psj.2021.101067 |
| Popis: | The enzyme 2′-5′ oligoadenylate synthase (OAS) is one of the key interferon-induced antiviral factors that act through inhibition of viral replication. In chickens, there is a single well-characterized OAS gene, oligoadenylate synthase-like (OASL) that has been shown to be upregulated after infection with various viruses. However, a deeper understanding of how chicken OASL acts against viral infection is still necessary. In this study, we tested the hypothesis that OASL short interfering RNA (siRNA)–mediated knockdown would decrease the host gene expression response to the Newcastle disease virus (NDV) by impacting antiviral pathways. To assess our hypothesis, a chicken fibroblast cell line (DF-1) was infected with the NDV (LaSota strain) and OASL expression was knocked down using a specific siRNA. The level of NDV viral RNA in the cells and the expression of interferon response- and apoptosis-related genes were evaluated by quantitative PCR at 4, 8, and 24 h postinfection (hpi). Knockdown of OASL increased the level of NDV viral RNA at 4, 8, and 24 hpi (P 0.05). The lack of differential expression suggests that knockdown of OASL resulted in a decreased response to NDV infection. Within NDV-infected cells, OASL knockdown reduced expression of signal transducer and activator of transcription 1, interferon alfa receptor subunit 1, eukaryotic translation initiation factor 2 alpha kinase 2, ribonuclease L, caspase 8 (CASP8) and caspase 9 (CASP9) at 4 hpi, CASP9 at 8 hpi, and caspase 3, CASP8, and CASP9 at 24 hpi (P |
| Databáze: | OpenAIRE |
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