A mutation in protein phosphatase 2A regulatory subunit A affects auxin transport in Arabidopsis
Autor: | P. Bernasconi, Christine Garbers, Dieter Söll, Jean Deruère, Alison DeLong |
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Rok vydání: | 1996 |
Předmět: |
Auxin efflux
DNA Complementary Molecular Sequence Data Mutant Phosphatase Arabidopsis Saccharomyces cerevisiae Regulatory Sequences Nucleic Acid Root hair General Biochemistry Genetics and Molecular Biology Auxin Phosphoprotein Phosphatases Amino Acid Sequence Protein Phosphatase 2 Cloning Molecular Molecular Biology Plant Proteins chemistry.chemical_classification Base Sequence Indoleacetic Acids General Immunology and Microbiology biology General Neuroscience fungi food and beverages Biological Transport Protein phosphatase 2 biology.organism_classification Cell biology Phenotype chemistry Biochemistry Mutation Basipetal auxin transport Research Article |
Zdroj: | The EMBO Journal. 15:2115-2124 |
ISSN: | 0261-4189 |
DOI: | 10.1002/j.1460-2075.1996.tb00565.x |
Popis: | The phytohormone auxin controls processes such as cell elongation, root hair development and root branching. Tropisms, growth curvatures triggered by gravity, light and touch, are also auxin-mediated responses. Auxin is synthesized in the shoot apex and transported through the stem, but the molecular mechanism of auxin transport is not well understood. Naphthylphthalamic acid (NPA) and other inhibitors of auxin transport block tropic curvature responses and inhibit root and shoot elongation. We have isolated a novel Arabidopsis thaliana mutant designated roots curl in NPA (rcn1). Mutant seedlings exhibit altered responses to NPA in root curling and hypocotyl elongation. Auxin efflux in mutant seedlings displays increased sensitivity to NPA. The rcn1 mutation was transferred-DNA (T-DNA) tagged and sequences flanking the T-DNA insert were cloned. Analysis of the RCN1 cDNA reveals that the T-DNA insertion disrupts a gene for the regulatory A subunit of protein phosphatase 2A (PP2A-A). The RCN1 gene rescues the rcn1 mutant phenotype and also complements the temperature-sensitive phenotype of the Saccharomyces cerevisiae PP2A-A mutation, tpd3-1. These data implicate protein phosphatase 2A in the regulation of auxin transport in Arabidopsis. |
Databáze: | OpenAIRE |
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