The Brain Link Protein-1 (BRAL1): cDNA Cloning, Genomic Structure, and Characterization as a Novel Link Protein Expressed in Adult Brain
| Autor: | Toshitaka Oohashi, Hidekatsu Yoshioka, Wei Dong Su, Takuro Murakami, Satoshi Hirakawa, Yoshifumi Ninomiya, Jirô Arata |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Aging Molecular Sequence Data Biophysics Nerve Tissue Proteins Biology Biochemistry Mice Exon Tandem repeat medicine Animals Humans Lectican Lectins C-Type Amino Acid Sequence RNA Messenger Cloning Molecular Hyaluronic Acid Brevican Molecular Biology Peptide sequence Aggrecan Extracellular Matrix Proteins Base Sequence Gene Expression Profiling Brain Gene Expression Regulation Developmental Proteins Exons Cell Biology Human brain Molecular biology Introns Protein Structure Tertiary Open reading frame medicine.anatomical_structure Chondroitin Sulfate Proteoglycans Proteoglycans Sequence Alignment |
| Zdroj: | Biochemical and Biophysical Research Communications. 276:982-989 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.2000.3583 |
| Popis: | We report here molecular cloning and expression analysis of the gene for a novel human brain link protein-1 (BRAL1) which is predominantly expressed in brain. The predicted open reading frame of human brain link protein-1 encoded a polypeptide of 340 amino acids containing three protein modules, the immunoglobulin-like fold and proteoglycan tandem repeat 1 and 2 domains, with an estimated mass of 38 kDa. The brain link protein-1 mRNA was exclusively present in brain. When analyzed during mouse development, it was detected solely in the adult brain. Concomitant expression pattern of mRNAs for brain link protein-1 and various lectican proteoglycans in brain suggests a possibility that brain link protein-1 functions to stabilize the binding between hyaluronan and brevican. The human BRAL1 gene contained 7 exons and spanned approximately 6 kb. The entire immunoglobulin-like fold was encoded by a single exon and the proteoglycan tandem repeat 1 and 2 domains were encoded by a single and two exons, respectively. The deduced amino acid sequence of human brain link protein-1 exhibited 45% identity with human cartilage link protein-1 (CRTL1), previously reported as link protein to stabilize aggregates of aggrecan and hyaluronan in cartilage. These results suggest that brain link protein-1 may have distinct function from cartilage link protein-1 and play specific roles, especially in the adult brain. |
| Databáze: | OpenAIRE |
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