The 7SK snRNP associates with the little elongation complex to promote snRNA gene expression
| Autor: | Patrice Vitali, Tamás Kiss, Michael Tellier, Sylvain Egloff, Raoul Raffel, Shona Murphy |
|---|---|
| Přispěvatelé: | Laboratoire de biologie moléculaire eucaryote (LBME), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre de Biologie Intégrative (CBI), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre de Biologie Intégrative (CBI), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Chromatin Immunoprecipitation [SDV]Life Sciences [q-bio] genetic processes information science RNA polymerase II Prp24 environment and public health General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine RNA Small Nuclear 7SK RNA Humans snRNP P-TEFb Molecular Biology ComputingMilieux_MISCELLANEOUS General Immunology and Microbiology biology urogenital system General Neuroscience RNA 7SK Small Nuclear RNA Articles Molecular biology 030104 developmental biology Gene Expression Regulation Ribonucleoproteins 030220 oncology & carcinogenesis health occupations biology.protein RNA Polymerase II Small nuclear RNA HeLa Cells Transcription Factors |
| Zdroj: | EMBO Journal EMBO Journal, EMBO Press, 2017, 36 (7), pp.934-948. ⟨10.15252/embj.201695740⟩ |
| ISSN: | 0261-4189 1460-2075 |
| DOI: | 10.15252/embj.201695740⟩ |
| Popis: | The 7SK small nuclear RNP (snRNP), composed of the 7SK small nuclear RNA (snRNA), MePCE, and Larp7, regulates the mRNA elongation capacity of RNA polymerase II (RNAPII) through controlling the nuclear activity of positive transcription elongation factor b (P-TEFb). Here, we demonstrate that the human 7SK snRNP also functions as a canonical transcription factor that, in collaboration with the little elongation complex (LEC) comprising ELL, Ice1, Ice2, and ZC3H8, promotes transcription of RNAPII-specific spliceosomal snRNA and small nucleolar RNA (snoRNA) genes. The 7SK snRNA specifically associates with a fraction of RNAPII hyperphosphorylated at Ser5 and Ser7, which is a hallmark of RNAPII engaged in snRNA synthesis. Chromatin immunoprecipitation (ChIP) and chromatin isolation by RNA purification (ChIRP) experiments revealed enrichments for all components of the 7SK snRNP on RNAPII-specific sn/snoRNA genes. Depletion of 7SK snRNA or Larp7 disrupts LEC integrity, inhibits RNAPII recruitment to RNAPII-specific sn/snoRNA genes, and reduces nascent snRNA and snoRNA synthesis. Thus, through controlling both mRNA elongation and sn/snoRNA synthesis, the 7SK snRNP is a key regulator of nuclear RNA production by RNAPII. |
| Databáze: | OpenAIRE |
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