HRP-mediated graft polymerization of acrylic acid onto silk fibroins and in situ biomimetic mineralization
| Autor: | Qian Zhou, Qiang Wang, Ping Wang, Yuanyuan Yu, Xuerong Fan, Jiugang Yuan, Chao Deng, Buguang Zhou |
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| Rok vydání: | 2018 |
| Předmět: |
Materials science
Biocompatibility Biomedical Engineering Biophysics Fibroin Biocompatible Materials Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Mineralization (biology) Bone and Bones Catalysis Polymerization Biomaterials chemistry.chemical_compound X-Ray Diffraction Biomimetics Cell Line Tumor Spectroscopy Fourier Transform Infrared Cell Adhesion Copolymer Animals Humans Particle Size Horseradish Peroxidase Acrylic acid Osteosarcoma Tissue Engineering Tissue Scaffolds fungi Temperature Hydrogen Peroxide Bombyx 021001 nanoscience & nanotechnology Grafting 0104 chemical sciences Durapatite Membrane Acrylates chemistry Thermogravimetry Microscopy Electron Scanning Stress Mechanical Fibroins 0210 nano-technology Nuclear chemistry |
| Zdroj: | Journal of Materials Science: Materials in Medicine. 29 |
| ISSN: | 1573-4838 0957-4530 |
| DOI: | 10.1007/s10856-018-6084-y |
| Popis: | Silk fibroin (SF) can be extensively utilized in biomedical areas owing to its appreciable bioactivity. In this study, biocompatible composites of SF and hydroxyapatite (HAp) were fabricated through in situ biomimetic mineralization process. Graft copolymerization of acrylic acid (AA) onto SF was conducted by using the catalytic system of acetylacetone (ACAC), hydrogen peroxide (H2O2) and horseradish peroxidase (HRP), for enhancing the deposition of apatite onto the fibroin chains. Subsequently, biomimetic mineralization of the prepared fibroin-based membrane was performed in Ca/P solutions to synthesize the organized SF/HAp composites. The efficacies of graft copolymerization and biomimetic mineralization were evaluated by means of ATR-FTIR, GPC, EDS-Mapping, XRD and others. The results denoted that AA was successfully graft-copolymerized with fibroin and formed the copolymer of silk fibroin-graft-polyacrylic acid (SF-g-PAA), and the grafting percentage (GP) and grafting efficiency (GE) under the optimal condition reached to 23.2% and 29.4%, respectively. More mineral phases were detected on the surface of SF-g-PAA membrane after mineralization process when compared to that of the untreated fibroin membrane, companying with an improved mechanical property. According to MG-63 cell viability and fluorescent adhesion assays, the mineralized SF-g-PAA composite showed satisfactory biocompatibility and exceptional adhesive effects as well. The synthetized composite of SF-g-PAA/HAp can be potentially applied in the fields of bone tissue engineering. |
| Databáze: | OpenAIRE |
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