Ribonuclease 7, an antimicrobial peptide upregulated during infection, contributes to microbial defense of the human urinary tract
| Autor: | Peter B. Baker, Sunder Sims-Lucas, Andrew L. Schwaderer, Julianne Bartz, Tad Eichler, David S. Hains, Jennifer Kline, John David Spencer, Kristin R. DeSouza, Huanyu Wang |
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| Rok vydání: | 2013 |
| Předmět: |
Sterility
RNase P Urinary system Antimicrobial peptides 030232 urology & nephrology Enzyme-Linked Immunosorbent Assay Nephron Biology Kidney Microscopy Atomic Force Real-Time Polymerase Chain Reaction Microbiology 03 medical and health sciences 0302 clinical medicine Ribonucleases medicine Humans RNA Messenger 030304 developmental biology 0303 health sciences Innate immune system Microbial Viability Bacteria Pyelonephritis Cell Membrane Antimicrobial Molecular biology 3. Good health Up-Regulation Kinetics medicine.anatomical_structure Nephrology Case-Control Studies Host-Pathogen Interactions Urinary Tract Infections |
| Zdroj: | Kidney International. 83(4):615-625 |
| ISSN: | 0085-2538 |
| DOI: | 10.1038/ki.2012.410 |
| Popis: | The mechanisms that maintain sterility in the urinary tract are incompletely understood; however, recent studies stress the importance of antimicrobial peptides in protecting the urinary tract from infection. Ribonuclease 7 (RNase 7), a potent antimicrobial peptide contributing to urinary tract sterility, is expressed by intercalated cells in the renal collecting tubules and is present in the urine at levels sufficient to kill bacteria at baseline. Here, we characterize the expression and function of RNase 7 in the human urinary tract during infection. Both quantitative real-time PCR and enzyme-linked immunosorbant assays demonstrated increases in RNASE7 expression in the kidney along with kidney and urinary RNase 7 peptide concentrations with infection. While immunostaining localized RNase 7 production to the intercalated cells of the collecting tubule during sterility, its expression during pyelonephritis was found to increase throughout the nephron but not in glomeruli or the interstitium. Recombinant RNase 7 exhibited antimicrobial activity against uropathogens at low micromolar concentrations by disrupting the microbial membrane as determined by atomic force microscopy. Thus, RNase 7 expression is increased in the urinary tract with infection and has antibacterial activity against uropathogens at micromolar concentrations. |
| Databáze: | OpenAIRE |
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