Effect of tranilast on proliferation, collagen gel contraction, and transforming growth factor beta secretion of retinal pigment epithelial cells and fibroblasts
| Autor: | Tsutomu Yasukawa, Yuichiro Ogura, Yasuhiko Tabata, Yoshihito Honda, Jin Dong, Hideya Kimura, Hideki Miyamoto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
pigmented rabbit
medicine.medical_specialty Proliferative vitreoretinopathy Time Factors Tranilast Biology proliferative vitreoretinopathy Cellular and Molecular Neuroscience chemistry.chemical_compound Pigment Transforming Growth Factor beta Internal medicine medicine Animals drug delivery system ortho-Aminobenzoates Transforming growth factor-beta secretion Pigment Epithelium of Eye Cells Cultured Skin Dose-Response Relationship Drug collagen gel contraction Retinal General Medicine Fibroblasts medicine.disease tranilast Molecular biology eye diseases Sensory Systems In vitro Ophthalmology Endocrinology chemistry visual_art visual_art.visual_art_medium Histamine H1 Antagonists Collagen gel contraction Cattle sense organs Collagen Rabbits Gels Cell Division medicine.drug |
| Zdroj: | OPHTHALMIC RESEARCH. 34(4):206-212 |
| ISSN: | 0030-3747 |
| Popis: | The efficiency of tranilast for the treatment of proliferative vitreoretinopathy (PVR) was investigated in vitro. A tetrazolium-based colorimetric assay showed that the 300-µM concentration of tranilast inhibited proliferation of bovine retinal pigment epithelial (RPE) cells and rabbit dermal fibroblasts with no toxicity. The contraction of collagen gels embedded with these cells was evaluated in the cultures. Compared with the gel incubated with minimal essential medium and 0.35% bovine serum albumin and/or fetal calf serum, tranilast inhibited gel contraction. Enzyme-linked immunosorbent assay revealed that a 300-µM concentration of tranilast inhibited transforming growth factor-β1 (TGF-β1) secretion significantly (p < 0.01). These results suggest that tranilast may inhibit the proliferation of RPE cells and fibroblasts and contraction of intraocular fibrous membranes by suppressing TGF-β1 secretion from these cells with a potential to treat PVR. |
| Databáze: | OpenAIRE |
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