Biochemical and Molecular Mechanisms Associated With the Resistance of the European Corn Borer (Lepidoptera: Crambidae) to Lambda-Cyhalothrin and First Monitoring Tool
| Autor: | Myriam Siegwart, Céline Hirn, Jean-Baptiste Thibord, Sandrine Maugin, Jérôme Olivares, Jan Elias, Claire Lavigne |
|---|---|
| Přispěvatelé: | Unité de recherche Plantes et Systèmes de Culture Horticoles (PSH), Institut National de la Recherche Agronomique (INRA), ARVALIS - Institut du végétal [Paris], Syngenta |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Insecticides Veterinary medicine European corn borer [SDV]Life Sciences [q-bio] deltaméthrine Polymerase Chain Reaction 01 natural sciences Carboxylesterase Ostrinia Insecticide Resistance chemistry.chemical_compound Cytochrome P-450 Enzyme System Crambidae Pyrethrins Glutathione Transferase education.field_of_study Ecology biology pyrethroid General Medicine cytochrome p450 (MFO) Lepidoptera mécanisme biochimique lambda cyhalothrine glutathion-s-transférase kdr Insect Proteins France Polymorphism Restriction Fragment Length pyrale du mais cytochrome Population Zea mays Lepidoptera genitalia 03 medical and health sciences Nitriles Botany Animals education deltamethrin biology.organism_classification Cyhalothrin 010602 entomology 030104 developmental biology Deltamethrin chemistry Insect Science Mutation PEST analysis mécanique moléculaire glutathione-S-transferase (GST) crambidae |
| Zdroj: | Journal of Economic Entomology Journal of Economic Entomology, Entomological Society of America, 2017, 110 (2), pp.598-606. ⟨10.1093/jee/tow267⟩ |
| ISSN: | 0022-0493 |
| DOI: | 10.1093/jee/tow267⟩ |
| Popis: | The European corn borer ( Ostrinia nubilalis (Hubner)) is one of the most serious corn pest in Europe where it is controlled with pesticides, in particular, pyrethroids. First control failures with this chemical family occurred on the field in 2008 in the center of France, and the first resistance case was described in 2012. In the present study, we investigate resistance mechanisms involved in seven French populations of O. nubilalis collected in the field. Resistances to deltamethrin and lambda-cyhalothrin were confirmed, with a higher resistance ratio for lambda-cyhalothrin (63.79 compared to 7.67). Resistance to the two active compounds was correlated except for one population, indicating a high probability of cross-resistance. Analyses of the activity of three major families of detoxification enzymes in resistant individuals showed a significant increase of the average MFO activity in males of four populations (activity ratios of 2.76–5.73) and higher GST activity in females of two other populations (activity ratios 4.48 and 5.21). Molecular investigation of the sodium channel gene sequence showed the presence of the kdr mutation in a highly resistant individual. We designed a PCR-RFLP screening tool to search for this mutation in the field, and we found it in five populations but not in the susceptible one. The resistance of O. nubilalis to pyrethroids in France seems to result from a combination of resistance mechanisms, possibly as a consequence of a selection pressure with an exceptional duration (almost 40 yr old). |
| Databáze: | OpenAIRE |
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