Response of cerebral endothelial cells to hypoxia: modification by fructose-1,6-bisphosphate but not glutamate receptor antagonists
| Autor: | Glenn T. Gobbel, George A. Gregory, Pak H. Chan, Thelma Y.-Y. Chan |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
medicine.medical_specialty Fructose 1 6-bisphosphate Central nervous system Ischemia Hypoglycemia Biology Rats Sprague-Dawley chemistry.chemical_compound Adenosine Triphosphate Ethidium Lactate dehydrogenase Internal medicine Fructosediphosphates medicine Animals Hypoxia Molecular Biology L-Lactate Dehydrogenase Staining and Labeling General Neuroscience Glutamate receptor Hypoxia (medical) medicine.disease Rats Endothelial stem cell medicine.anatomical_structure Endocrinology chemistry Cerebrovascular Circulation Female Endothelium Vascular Neurology (clinical) medicine.symptom Excitatory Amino Acid Antagonists Developmental Biology |
| Zdroj: | Brain Research. 653:23-30 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/0006-8993(94)90367-0 |
| Popis: | Damage to the cerebral endothelium from ischemia could exacerbate brain injury by altering vascular integrity, but little is known concerning the response of cerebral endothelial cells to hypoxia. To address this issue, cerebral capillary endothelial cells were isolated from 14-day-old rats, grown to confluence, and placed in hypoxic chambers for up to 62 h. Cells were undamaged by 24 hours of hypoxia as assessed by lactate dehydrogenase release and ethidium bromide staining, but 48 h resulted in marked damage. Hypoxia was probably exacerbated by hypoglycemia because glucose levels fell to1 mM by 24 h, at which point ATP levels began to fall in hypoxic cultures (3.25 +/- 1.48 nmol/mg protein; mean +/- S.D.) relative to normoxic cultures (9.52 +/- 1.41 nmol/mg protein). Cells treated with 4 mM fructose-1,6-bisphosphate (FBP) had significantly less damage at 48 h of hypoxia than controls. FBP had little effect on rate of glucose depletion from the media, but ATP depletion due to hypoxia was significantly less. Thus, the protective effect of FBP may be mediated by the ability of treated cells to maintain higher ATP levels. Unlike FBP, glutamate receptor antagonists including MK-801, NBQX, DNQX, and kynurenic acid were ineffective in ameliorating hypoxia-induced endothelial cell injury. |
| Databáze: | OpenAIRE |
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